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PMID: 21502492 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Immune escape from HIV-specific antibody-dependent cellular cytotoxicity (ADCC) pressure.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 108 ·No. 18 ·2011-05-03 ·Pages 7505-10

Chung AW, Isitman G, Navis M, Kramski M, Center RJ, Kent SJ, Stratov I

Abstract

Effective immunity to HIV is poorly understood. In particular, a role for antibody-dependent cellular cytotoxicity (ADCC) in controlling HIV is controversial. We hypothesized that significant pressure from HIV-specific ADCC would result in immune-escape variants. A series of ADCC epitopes in HIV-infected subjects to specific consensus strain HIV peptides were mapped using a flow cytometric assay for natural killer cell activation. We then compared the ADCC responses to the same peptide epitope derived from the concurrent HIV sequence(s) expressed in circulating virus. In 9 of 13 epitopes studied, ADCC antibodies were unable to recognize the concurrent HIV sequence. Our studies suggest ADCC responses apply significant immune pressure on the virus. This result has implications for the induction of ADCC responses by HIV vaccines.

MeSH Terms
Antibody-Dependent Cell Cytotoxicity/genetics,immunology Base Sequence Epitope Mapping Epitopes/genetics,immunology Flow Cytometry Gene Products, env/genetics HIV Infections/immunology HIV-1/immunology Humans Killer Cells, Natural/immunology Lymphocyte Activation/immunology Molecular Sequence Data Neutralization Tests Plasmids/genetics Sequence Analysis, DNA
Chemicals
Epitopes Gene Products, env
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Chung Amy W
Department of Microbiology and Immunology, University of Melbourne, Parkville, VIC 3010, Australia.
Isitman Gamze
Navis Marjon
Kramski Marit
Center Rob J
Kent Stephen J
Stratov Ivan
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.1016048108
Published
2011-05-03
Epub
2011-00-18
Pages
7505-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC3088575
Subset
IM
Grants
NIAID NIH HHS · R21 AI081541 · United States
NIAID NIH HHS · R21AI081541 · United States
External Links
PubMed source DOI: 10.1073/pnas.1016048108 PMC Full Text View journal details
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