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PMID: 21497118 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Asymmetric proteasome segregation as a mechanism for unequal partitioning of the transcription factor T-bet during T lymphocyte division.

Immunity ·Vol. 34 ·No. 4 ·2011-04-22 ·Pages 492-504

Chang JT, Ciocca ML, Kinjyo I, Palanivel VR, McClurkin CE, Dejong CS, Mooney EC, Kim JS, Steinel NC, Oliaro J, Yin CC, Florea BI, Overkleeft HS, Berg LJ, Russell SM, Koretzky GA, Jordan MS, Reiner SL

Abstract

Polarized segregation of proteins in T cells is thought to play a role in diverse cellular functions including signal transduction, migration, and directed secretion of cytokines. Persistence of this polarization can result in asymmetric segregation of fate-determining proteins during cell division, which may enable a T cell to generate diverse progeny. Here, we provide evidence that a lineage-determining transcription factor, T-bet, underwent asymmetric organization in activated T cells preparing to divide and that it was unequally partitioned into the two daughter cells. This unequal acquisition of T-bet appeared to result from its asymmetric destruction during mitosis by virtue of concomitant asymmetric segregation of the proteasome. These results suggest a mechanism by which a cell may unequally localize cellular activities during division, thereby imparting disparity in the abundance of cell fate regulators in the daughter cells.

MeSH Terms
Animals Cell Polarity Cells, Cultured Mice Mice, Inbred C57BL Mitosis Phosphorylation Proteasome Endopeptidase Complex/metabolism T-Box Domain Proteins/immunology,metabolism T-Lymphocytes/cytology,enzymology,immunology
Chemicals
T-Box Domain Proteins T-box transcription factor TBX21 Proteasome Endopeptidase Complex
Authors & Affiliations
18 authors, click to expand affiliations / ORCID
Chang John T
Abramson Family Cancer Research Institute and Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. changj@ucsd.edu
Ciocca Maria L
Kinjyo Ichiko
Palanivel Vikram R
McClurkin Courtney E
Dejong Caitlin S
Mooney Erin C
Kim Jiyeon S
Steinel Natalie C
Oliaro Jane
Yin Catherine C
Florea Bogdan I
Overkleeft Herman S
Berg Leslie J
Russell Sarah M
Koretzky Gary A
Jordan Martha S
Reiner Steven L
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Article Info
Journal
Immunity
Abbr.
Immunity
ISSN
1097-4180
DOI
10.1016/j.immuni.2011.03.017
Email
changj@ucsd.edu
Published
2011-04-22
Epub
2011-00-14
Pages
492-504
Language
English
Region
United States
NLM ID
9432918
PMCID
PMC3088519
Subset
IM
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NIAMS NIH HHS · K01 AR052802 · United States
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NIAID NIH HHS · R01 AI061699-05 · United States
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NIAID NIH HHS · R01 AI046564-04 · United States
NIGMS NIH HHS · R37 GM053256 · United States
NIDDK NIH HHS · K08 DK080949-05 · United States
NIAMS NIH HHS · AR052802 · United States
NIAMS NIH HHS · K01 AR052802-05 · United States
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NIAID NIH HHS · AI042370 · United States
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CommentIn
PMID: 21597476
External Links
PubMed source DOI: 10.1016/j.immuni.2011.03.017 PMC Full Text View journal details
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