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PMID: 21467579 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A sister group contrast using untargeted global metabolomic analysis delineates the biochemical regulation underlying desiccation tolerance in Sporobolus stapfianus.

The Plant cell ·Vol. 23 ·No. 4 ·2011-04-00 ·Pages 1231-48

Oliver MJ, Guo L, Alexander DC, Ryals JA, Wone BW, Cushman JC

Abstract

Understanding how plants tolerate dehydration is a prerequisite for developing novel strategies for improving drought tolerance. The desiccation-tolerant (DT) Sporobolus stapfianus and the desiccation-sensitive (DS) Sporobolus pyramidalis formed a sister group contrast to reveal adaptive metabolic responses to dehydration using untargeted global metabolomic analysis. Young leaves from both grasses at full hydration or at 60% relative water content (RWC) and from S. stapfianus at lower RWCs were analyzed using liquid and gas chromatography linked to mass spectrometry or tandem mass spectrometry. Comparison of the two species in the fully hydrated state revealed intrinsic differences between the two metabolomes. S. stapfianus had higher concentrations of osmolytes, lower concentrations of metabolites associated with energy metabolism, and higher concentrations of nitrogen metabolites, suggesting that it is primed metabolically for dehydration stress. Further reduction of the leaf RWC to 60% instigated a metabolic shift in S. stapfianus toward the production of protective compounds, whereas S. pyramidalis responded differently. The metabolomes of S. stapfianus leaves below 40% RWC were strongly directed toward antioxidant production, nitrogen remobilization, ammonia detoxification, and soluble sugar production. Collectively, the metabolic profiles obtained uncovered a cascade of biochemical regulation strategies critical to the survival of S. stapfianus under desiccation.

MeSH Terms
Adaptation, Physiological Allantoin/metabolism Asparagine/metabolism Citric Acid Cycle Desiccation Glutamine/metabolism Glutathione/biosynthesis Glycolysis Metabolome Metabolomics/methods Nitrogen/metabolism Phenotype Poaceae/metabolism Raffinose/metabolism Tocopherols/metabolism Water
Chemicals
Water Glutamine Allantoin Asparagine Glutathione Raffinose Nitrogen Tocopherols
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Oliver Melvin J
U.S. Department of Agriculture-Agricultural Research Service, Plant Genetic Research Unit, University of Missouri, Columbia, Missouri 65211, USA. mel.oliver@ars.usda.gov
Guo Lining
Alexander Danny C
Ryals John A
Wone Bernard W M
Cushman John C
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.110.082800
Email
mel.oliver@ars.usda.gov
Published
2011-04-00
Epub
2011-00-05
Pages
1231-48
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3101564
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.110.082800 PMC Full Text View journal details
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