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PMID: 2142162 Published · ppublish English Journal Article

Regulation of immunoreactive GAP-43 expression in rat cortical macroglia is cell type specific.

The Journal of cell biology ·Vol. 111 ·No. 1 ·1990-07-00 ·Pages 209-15

da Cunha A, Vitković L

Abstract

Growth-associated protein 43 (GAP-43) is an abundant, intensely investigated membrane phosphoprotein of the nervous system (Benowitz, L.I., and A. Routtenberg. 1987. Trends Neurosci. 10:527-532; Skene, J. H. P. 1989. Annu. Rev. Neurosci. 12:127-156), with a hitherto unknown function. We have previously demonstrated that astrocytes, brain macroglial cells, contain GAP-43 (Steisslinger, H. W., V. J. Aloyo, and L. Vitković, 1987. Brain Res. 415:375-379; Vitković, L., H. W. Steisslinger, V. J. Aloyo, and M. Mersel. 1988. Proc. Natl. Acad. Sci. USA. 85:8296-8300; Vitković L., and M. Mersel. 1989. Metab. Brain Dis. 4:47-53). Results from double immunofluorescent labeling experiments presented here show that oligodendrocytes also contain GAP-43 immunoreactivity (GAP-43ir). Thus, all three macroglial cell types of the central nervous system (type I and type 2 astrocytes and oligodendrocytes) contain GAP-43. Whereas immunoreactive GAP-43 is expressed by progenitors of all macroglial cell types, the developmental regulation of its expression is cell type specific. Immunoreactive GAP-43 is downregulated in type 1 astrocytes, and constitutively expressed in both type 2 astrocytes and oligodendrocytes. These results may be relevant to potential function(s) of GAP-43.

MeSH Terms
Animals Astrocytes/cytology,metabolism Cells, Cultured Cerebral Cortex/cytology,metabolism Fluorescein-5-isothiocyanate Fluoresceins Fluorescent Antibody Technique Fluorescent Dyes GAP-43 Protein Kinetics Membrane Glycoproteins/analysis,biosynthesis,immunology Nerve Tissue Proteins/analysis,biosynthesis,immunology Neurons/cytology,metabolism Oligodendroglia/cytology,metabolism Phosphoproteins/biosynthesis Rats Rats, Inbred Strains Thiocyanates Time Factors
Chemicals
Fluoresceins Fluorescent Dyes GAP-43 Protein Membrane Glycoproteins Nerve Tissue Proteins Phosphoproteins Thiocyanates Fluorescein-5-isothiocyanate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
da Cunha A
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Vitković L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.1.209
Published
1990-07-00
Pages
209-15
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116173
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.111.1.209 PMC Full Text View journal details
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