Home LiteratureArticle Details
PMID: 21398568 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The MYB96 transcription factor regulates cuticular wax biosynthesis under drought conditions in Arabidopsis.

The Plant cell ·Vol. 23 ·No. 3 ·2011-03-00 ·Pages 1138-52

Seo PJ, Lee SB, Suh MC, Park MJ, Go YS, Park CM

Abstract

Drought stress activates several defense responses in plants, such as stomatal closure, maintenance of root water uptake, and synthesis of osmoprotectants. Accumulating evidence suggests that deposition of cuticular waxes is also associated with plant responses to cellular dehydration. Yet, how cuticular wax biosynthesis is regulated in response to drought is unknown. We have recently reported that an Arabidopsis thaliana abscisic acid (ABA)-responsive R2R3-type MYB transcription factor, MYB96, promotes drought resistance. Here, we show that transcriptional activation of cuticular wax biosynthesis by MYB96 contributes to drought resistance. Microarray assays showed that a group of wax biosynthetic genes is upregulated in the activation-tagged myb96-1D mutant but downregulated in the MYB96-deficient myb96-1 mutant. Cuticular wax accumulation was altered accordingly in the mutants. In addition, activation of cuticular wax biosynthesis by drought and ABA requires MYB96. By contrast, biosynthesis of cutin monomers was only marginally affected in the mutants. Notably, the MYB96 protein acts as a transcriptional activator of genes encoding very-long-chain fatty acid-condensing enzymes involved in cuticular wax biosynthesis by directly binding to conserved sequence motifs present in the gene promoters. These results demonstrate that ABA-mediated MYB96 activation of cuticular wax biosynthesis serves as a drought resistance mechanism.

MeSH Terms
Abscisic Acid/metabolism Acetyltransferases/genetics Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics,metabolism Chlorophyll/analysis Droughts Gene Expression Regulation, Plant Membrane Lipids/analysis Plant Leaves/metabolism,ultrastructure Plants, Genetically Modified/genetics,metabolism Promoter Regions, Genetic Transcription Factors/genetics,metabolism Water/metabolism Waxes/analysis,metabolism
Chemicals
Arabidopsis Proteins MYB96 protein, Arabidopsis Membrane Lipids Transcription Factors Waxes Water Chlorophyll cutin Abscisic Acid Acetyltransferases KCS2 protein, Arabidopsis
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Seo Pil Joon
Department of Chemistry, Seoul National University, Seoul 151-742, Korea.
Lee Saet Buyl
Suh Mi Chung
Park Mi-Jeong
Go Young Sam
Park Chung-Mo
References (74)
74 references, click to expand
  1. A membrane-bound NAC transcription factor regulates cell division in Arabidopsis.
    Plant Cell. 2006 Nov;18(11):3132-44 PMID: 17098812
  2. Arabidopsis CYP86A2 represses Pseudomonas syringae type III genes and is required for cuticle development.
    EMBO J. 2004 Jul 21;23(14):2903-13 PMID: 15241470
  3. Overexpression of WXP1, a putative Medicago truncatula AP2 domain-containing transcription factor gene, increases cuticular wax accumulation and enhances drought tolerance in transgenic alfalfa (Medicago sativa).
    Plant J. 2005 Jun;42(5):689-707 PMID: 15918883
  4. Unraveling the complex network of cuticular structure and function.
    Curr Opin Plant Biol. 2006 Jun;9(3):281-7 PMID: 16580871
  5. Genetic analysis of organ fusion in Arabidopsis thaliana.
    Genetics. 1998 Jun;149(2):607-19 PMID: 9611177
  6. CUT1, an Arabidopsis gene required for cuticular wax biosynthesis and pollen fertility, encodes a very-long-chain fatty acid condensing enzyme.
    Plant Cell. 1999 May;11(5):825-38 PMID: 10330468
  7. Significance analysis of microarrays applied to the ionizing radiation response.
    Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21 PMID: 11309499
  8. Guard cell abscisic acid signalling and engineering drought hardiness in plants.
    Nature. 2001 Mar 15;410(6826):327-30 PMID: 11268200
  9. Identification of the wax ester synthase/acyl-coenzyme A: diacylglycerol acyltransferase WSD1 required for stem wax ester biosynthesis in Arabidopsis.
    Plant Physiol. 2008 Sep;148(1):97-107 PMID: 18621978
  10. The MYB96 transcription factor mediates abscisic acid signaling during drought stress response in Arabidopsis.
    Plant Physiol. 2009 Sep;151(1):275-89 PMID: 19625633
  11. A putative hydroxysteroid dehydrogenase involved in regulating plant growth and development.
    Plant Physiol. 2007 Sep;145(1):87-97 PMID: 17616511
  12. The CER3 wax biosynthetic gene from Arabidopsis thaliana is allelic to WAX2/YRE/FLP1.
    FEBS Lett. 2007 Jul 24;581(18):3538-44 PMID: 17624331
  13. The DAISY gene from Arabidopsis encodes a fatty acid elongase condensing enzyme involved in the biosynthesis of aliphatic suberin in roots and the chalaza-micropyle region of seeds.
    Plant J. 2009 Jan;57(1):80-95 PMID: 18786002
  14. Optical properties and contribution of cuticle to UV protection in plants: experiments with apple fruit.
    Photochem Photobiol Sci. 2003 Aug;2(8):861-6 PMID: 14521223
  15. The Arabidopsis DESPERADO/AtWBC11 transporter is required for cutin and wax secretion.
    Plant Physiol. 2007 Dec;145(4):1345-60 PMID: 17951461
  16. The acyl-CoA synthetase encoded by LACS2 is essential for normal cuticle development in Arabidopsis.
    Plant Cell. 2004 Mar;16(3):629-42 PMID: 14973169
  17. Mimicking the plant cell interior under water stress by macromolecular crowding: disordered dehydrin proteins are highly resistant to structural collapse.
    Plant Physiol. 2008 Dec;148(4):1925-37 PMID: 18849483
  18. An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence.
    Plant Cell. 1993 Nov;5(11):1529-39 PMID: 8312738
  19. Very-long-chain fatty acid biosynthesis is controlled through the expression and specificity of the condensing enzyme.
    Plant J. 1997 Jul;12(1):121-31 PMID: 9263455
  20. Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses.
    Annu Rev Plant Biol. 2006;57:781-803 PMID: 16669782
  21. Cytological and biochemical analysis of COF1, an Arabidopsis mutant of an ABC transporter gene.
    Plant Cell Physiol. 2007 Nov;48(11):1524-33 PMID: 17971336
  22. MYB96-mediated abscisic acid signals induce pathogen resistance response by promoting salicylic acid biosynthesis in Arabidopsis.
    New Phytol. 2010 Apr;186(2):471-83 PMID: 20149112
  23. The cytochrome P450 enzyme CYP96A15 is the midchain alkane hydroxylase responsible for formation of secondary alcohols and ketones in stem cuticular wax of Arabidopsis.
    Plant Physiol. 2007 Nov;145(3):653-67 PMID: 17905869
  24. A concerted DNA methylation/histone methylation switch regulates rRNA gene dosage control and nucleolar dominance.
    Mol Cell. 2004 Feb 27;13(4):599-609 PMID: 14992728
  25. Regulatory metabolic networks in drought stress responses.
    Curr Opin Plant Biol. 2007 Jun;10(3):296-302 PMID: 17468040
  26. CER4 encodes an alcohol-forming fatty acyl-coenzyme A reductase involved in cuticular wax production in Arabidopsis.
    Plant Physiol. 2006 Nov;142(3):866-77 PMID: 16980563
  27. Molecular genetic perspectives on cross-talk and specificity in abiotic stress signalling in plants.
    J Exp Bot. 2004 Jan;55(395):225-36 PMID: 14673035
  28. The transcription factor WIN1/SHN1 regulates Cutin biosynthesis in Arabidopsis thaliana.
    Plant Cell. 2007 Apr;19(4):1278-94 PMID: 17449808
  29. Changes in leaf cuticular waxes of sesame (Sesamum indicum L.) plants exposed to water deficit.
    J Plant Physiol. 2007 Sep;164(9):1134-43 PMID: 16904233
  30. Integration of abscisic acid signalling into plant responses.
    Plant Biol (Stuttg). 2006 May;8(3):314-25 PMID: 16807823
  31. Molecular characterization of the CER1 gene of arabidopsis involved in epicuticular wax biosynthesis and pollen fertility.
    Plant Cell. 1995 Dec;7(12):2115-27 PMID: 8718622
  32. Microbial biosynthesis of alkanes.
    Science. 2010 Jul 30;329(5991):559-62 PMID: 20671186
  33. The guard cell as a single-cell model towards understanding drought tolerance and abscisic acid action.
    J Exp Bot. 2009;60(5):1439-63 PMID: 19181866
  34. Arabidopsis genes involved in acyl lipid metabolism. A 2003 census of the candidates, a study of the distribution of expressed sequence tags in organs, and a web-based database.
    Plant Physiol. 2003 Jun;132(2):681-97 PMID: 12805597
  35. Physiological Effects of Surface Waxes: I. Light Reflectance for Glaucous and Nonglaucous Picea pungens.
    Plant Physiol. 1978 Jul;62(1):101-4 PMID: 16660444
  36. Arabidopsis PLC1 is required for secondary responses to abscisic acid signals.
    Plant Cell. 2001 May;13(5):1143-54 PMID: 11340187
  37. Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter.
    Plant Cell. 2004 Sep;16(9):2481-98 PMID: 15319476
  38. Cuticular lipid composition, surface structure, and gene expression in Arabidopsis stem epidermis.
    Plant Physiol. 2005 Dec;139(4):1649-65 PMID: 16299169
  39. Increased accumulation of cuticular wax and expression of lipid transfer protein in response to periodic drying events in leaves of tree tobacco.
    Plant Physiol. 2006 Jan;140(1):176-83 PMID: 16361524
  40. A MYB transcription factor regulates very-long-chain fatty acid biosynthesis for activation of the hypersensitive cell death response in Arabidopsis.
    Plant Cell. 2008 Mar;20(3):752-67 PMID: 18326828
  41. Heterologous expression of two Medicago truncatula putative ERF transcription factor genes, WXP1 and WXP2, in Arabidopsis led to increased leaf wax accumulation and improved drought tolerance, but differential response in freezing tolerance.
    Plant Mol Biol. 2007 Jun;64(3):265-78 PMID: 17347795
  42. Bioconductor: open software development for computational biology and bioinformatics.
    Genome Biol. 2004;5(10):R80 PMID: 15461798
  43. The impact of water deficiency on leaf cuticle lipids of Arabidopsis.
    Plant Physiol. 2009 Dec;151(4):1918-29 PMID: 19819982
  44. Salt and drought stress signal transduction in plants.
    Annu Rev Plant Biol. 2002;53:247-73 PMID: 12221975
  45. Functional analysis of the LACERATA gene of Arabidopsis provides evidence for different roles of fatty acid omega -hydroxylation in development.
    Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9694-9 PMID: 11493698
  46. Characterization of Arabidopsis ABCG11/WBC11, an ATP binding cassette (ABC) transporter that is required for cuticular lipid secretion.
    Plant J. 2007 Nov;52(3):485-98 PMID: 17727615
  47. Technical advance: An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants.
    Plant J. 2000 Oct;24(2):265-73 PMID: 11069700
  48. Two Arabidopsis 3-ketoacyl CoA synthase genes, KCS20 and KCS2/DAISY, are functionally redundant in cuticular wax and root suberin biosynthesis, but differentially controlled by osmotic stress.
    Plant J. 2009 Nov;60(3):462-75 PMID: 19619160
  49. Protecting against water loss: analysis of the barrier properties of plant cuticles.
    J Exp Bot. 2001 Oct;52(363):2023-32 PMID: 11559738
  50. Sealing plant surfaces: cuticular wax formation by epidermal cells.
    Annu Rev Plant Biol. 2008;59:683-707 PMID: 18251711
  51. Acyl-lipid metabolism.
    Arabidopsis Book. 2010;8:e0133 PMID: 22303259
  52. An ABC transporter gene of Arabidopsis thaliana, AtWBC11, is involved in cuticle development and prevention of organ fusion.
    Plant Cell Physiol. 2007 Dec;48(12):1790-802 PMID: 17989085
  53. Alterations in CER6, a gene identical to CUT1, differentially affect long-chain lipid content on the surface of pollen and stems.
    Plant Cell. 2000 Oct;12(10):2001-8 PMID: 11041893
  54. KCS1 encodes a fatty acid elongase 3-ketoacyl-CoA synthase affecting wax biosynthesis in Arabidopsis thaliana.
    Plant J. 1999 Jan;17(2):119-30 PMID: 10074711
  55. A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway.
    Plant J. 2004 Sep;39(6):863-76 PMID: 15341629
  56. Plant cuticles shine: advances in wax biosynthesis and export.
    Curr Opin Plant Biol. 2009 Dec;12(6):721-7 PMID: 19864175
  57. Plant surface lipid biosynthetic pathways and their utility for metabolic engineering of waxes and hydrocarbon biofuels.
    Plant J. 2008 May;54(4):670-83 PMID: 18476871
  58. SIZ1-mediated sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance in Arabidopsis.
    Plant Cell. 2007 Apr;19(4):1403-14 PMID: 17416732
  59. Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling.
    Plant Cell. 2002 Feb;14(2):343-57 PMID: 11884679
  60. Disruption of glycosylphosphatidylinositol-anchored lipid transfer protein gene altered cuticular lipid composition, increased plastoglobules, and enhanced susceptibility to infection by the fungal pathogen Alternaria brassicicola.
    Plant Physiol. 2009 May;150(1):42-54 PMID: 19321705
  61. The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis.
    Plant Cell. 2004 Sep;16(9):2463-80 PMID: 15319479
  62. Significance of the expression of the CER6 condensing enzyme for cuticular wax production in Arabidopsis.
    Plant Physiol. 2002 Aug;129(4):1568-80 PMID: 12177469
  63. The effect of wax components on cuticular transpiration-model experiments.
    Planta. 1967 Mar;75(1):23-7 PMID: 24550011
  64. Regulatory network of gene expression in the drought and cold stress responses.
    Curr Opin Plant Biol. 2003 Oct;6(5):410-7 PMID: 12972040
  65. Biosynthesis and secretion of plant cuticular wax.
    Prog Lipid Res. 2003 Jan;42(1):51-80 PMID: 12467640
  66. Cloning and characterization of the WAX2 gene of Arabidopsis involved in cuticle membrane and wax production.
    Plant Cell. 2003 May;15(5):1170-85 PMID: 12724542
  67. Over-expression of the Arabidopsis AtMYB41 gene alters cell expansion and leaf surface permeability.
    Plant J. 2008 Jan;53(1):53-64 PMID: 17971045
  68. The VLCFA elongase gene family in Arabidopsis thaliana: phylogenetic analysis, 3D modelling and expression profiling.
    Plant Mol Biol. 2008 Jul;67(5):547-66 PMID: 18465198
  69. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling.
    Plant Cell. 2003 Jan;15(1):63-78 PMID: 12509522
  70. The HIC signalling pathway links CO2 perception to stomatal development.
    Nature. 2000 Dec 7;408(6813):713-6 PMID: 11130071
  71. BIOCHEMISTRY AND MOLECULAR BIOLOGY OF WAX PRODUCTION IN PLANTS.
    Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:405-430 PMID: 15012295
  72. Functional characterization of the Arabidopsis beta-ketoacyl-coenzyme A reductase candidates of the fatty acid elongase.
    Plant Physiol. 2009 Jul;150(3):1174-91 PMID: 19439572
  73. Building lipid barriers: biosynthesis of cutin and suberin.
    Trends Plant Sci. 2008 May;13(5):236-46 PMID: 18440267
  74. Plant cuticular lipid export requires an ABC transporter.
    Science. 2004 Oct 22;306(5696):702-4 PMID: 15499022
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.111.083485
Published
2011-03-00
Epub
2011-00-11
Pages
1138-52
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3082259
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.111.083485 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2