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题目:: Mouse cytolytic T cells reactive with rat islet tumour RIN 5AH-B cells.
作者:: Schøller(J),Rubin(B)
状态:: 发布时间1990-05-02 , 更新时间 2015-11-19
期刊:: APMIS
摘要:: Insulin dependent (type 1) diabetes mellitus is considered to be an autoimmune disease characterized by a specific destruction of the insulin-producing pancreatic beta cells. We have explored the possibilities of raising T cells specific for putative pancreatic beta-cell autoantigens using a xenogeneic system. Mouse T cells were induced against the rat insulinoma cells RIN 5AH-B (RIN) and tested for their specific reactivity. No MHC class II-restricted beta-cell-specific helper T-cell reactivity could be detected within the bulk cultures as measured by proliferation, but a remarkably high cytotoxicity against the RIN cells was observed. The target antigen on the cell surface recognized by the generated cytotoxic T cells was shown to be the rat class I major histocompatibility antigen RT1g, and not a beta-cell or tumour cell-specific antigen associated with RIN cell MHC molecules. Our results demonstrate that it is feasible to evoke a xenogeneic T-cell response against the RIN cells. However, the mouse T cells recognize a dominant epitope present on the expressed rat class I major histocompatibility antigen RT1Ag and not a beta-cell-specific antigen. Hence, we conclude that it appears most unlikely that beta-cell-specific T cells can be raised in the xenogeneic system.
语言:: eng
DOI:

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