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PMID: 2137856 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Intracellular application of guanosine-5'-O-(3-thiotriphosphate) induces exocytotic granule fusion in guinea pig eosinophils.

The Journal of experimental medicine ·Vol. 171 ·No. 3 ·1990-03-01 ·Pages 775-86

Nüsse O, Lindau M, Cromwell O, Kay AB, Gomperts BD

Abstract

The mechanism of eosinophil secretion was studied in guinea pig eosinophils by measuring release of hexosaminidase from cell suspensions (greater than 98% pure) permeabilized with streptolysin-O and by whole-cell patch-clamp capacitance measurements. It is shown that release of eosinophil granule components occurs by an exocytotic mechanism in which individual granules fuse with the plasma membrane. Exocytosis can be induced by intracellular application of the nonhydrolyzable GTP analog guanosine-5'-O-(3-thiotriphosphate) (GTP-gamma-S), suggesting the involvement of a GTP-binding protein. The activation is modulated by the intracellular calcium concentration, with activation by GTP-gamma-S inducing transient elevations in the concentration of Ca2+. Thus, the nature and regulation of the release mechanism appear to be very similar to that of the mast cell and neutrophil.

MeSH Terms
Animals Calcium/pharmacology Cell Degranulation/drug effects Cytoplasmic Granules/drug effects Eosinophils/drug effects,metabolism Exocytosis/drug effects Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Triphosphate/analogs & derivatives,pharmacology Guinea Pigs Mast Cells/drug effects,metabolism Thionucleotides/pharmacology beta-N-Acetylhexosaminidases/metabolism
Chemicals
Thionucleotides Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Triphosphate beta-N-Acetylhexosaminidases Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nüsse O
Biophysics Group, Freie Universität Berlin, Federal Republic of Germany.
Lindau M
Cromwell O
Kay A B
Gomperts B D
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.171.3.775
Published
1990-03-01
Pages
775-86
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187767
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1084/jem.171.3.775 PMC Full Text View journal details
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