Interleukin-1 receptor antagonist activity of a human interleukin-1 inhibitor.

Nature ·Vol. 343 ·No. 6256 ·1990-01-25 ·Pages 336-40

Hannum CH, Wilcox CJ, Arend WP, Joslin FG, Dripps DJ, Heimdal PL, Armes LG, Sommer A, Eisenberg SP, Thompson RC

Abstract

Three interleukin-1 inhibitors have been purified to homogeneity from medium conditioned by human monocytes. Partial sequence analysis and digestion with N-glycanase indicate that these are glycosylation forms of a single protein. The protein binds to the interleukin-1 receptor but has no interleukin-1-like activity, even at very high concentrations, and is therefore a pure receptor antagonist.

MeSH Terms

Amidohydrolases Amino Acid Sequence Binding, Competitive Cells, Cultured Chromatography Chromatography, High Pressure Liquid Dinoprostone/biosynthesis Electrophoresis, Polyacrylamide Gel Fibroblasts/metabolism Glycosylation Humans Interleukin 1 Receptor Antagonist Protein Interleukin-1/antagonists & inhibitors Molecular Sequence Data Monocytes/metabolism Peptide Fragments Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase Proteins/isolation & purification,metabolism,pharmacology Receptors, Immunologic/antagonists & inhibitors,metabolism Receptors, Interleukin-1 Recombinant Proteins/metabolism,pharmacology Sialoglycoproteins

Chemicals

IL1RN protein, human Interleukin 1 Receptor Antagonist Protein Interleukin-1 Peptide Fragments Proteins Receptors, Immunologic Receptors, Interleukin-1 Recombinant Proteins Sialoglycoproteins Amidohydrolases Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase Dinoprostone

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Hannum C H

Synergen Inc., Boulder, Colorado 80301.

Wilcox C J

Arend W P

Joslin F G

Dripps D J

Heimdal P L

Armes L G

Sommer A

Eisenberg S P

Thompson R C

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/343336a0

Published

1990-01-25

Pages

336-40

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/343336a0 View journal details

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Interleukin-1 receptor antagonist activity of a human interleukin-1 inhibitor.

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