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PMID: 21337465 Published · ppublish English Journal Article Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't

Stem cell marker prominin-1 regulates branching morphogenesis, but not regenerative capacity, in the mammary gland.

Developmental dynamics : an official publication of the American Association of Anatomists ·Vol. 240 ·No. 3 ·2011-03-00 ·Pages 674-81

Anderson LH, Boulanger CA, Smith GH, Carmeliet P, Watson CJ

Abstract

Prominin-1 (Prom1) is recognized as a stem cell marker in several tissues, including blood, neuroepithelium, and gut, and in human and mouse embryos and many cancers. Although Prom1 is routinely used as a marker for isolating stem cells, its biological function remains unclear. Here we use a knockout model to investigate the role of Prom1 in the mammary gland. We demonstrate that complete loss of Prom1 does not affect the regenerative capacity of the mammary epithelium. Surprisingly, we also show that in the absence of Prom1, mammary glands have reduced ductal branching, and an increased ratio of luminal to basal cells. The effects of Prom1 loss in the mammary gland are associated with decreased expression of prolactin receptor and matrix metalloproteinase-3. These experiments reveal a novel, functional role for Prom1 that is not related to stem cell activity, and demonstrate the importance of tissue-specific characterization of putative stem cell markers.

MeSH Terms
AC133 Antigen Animals Antigens, CD/genetics,metabolism Female Flow Cytometry Glycoproteins/genetics,metabolism Mammary Glands, Animal/cytology,metabolism,physiology Mice Mice, Mutant Strains Mice, Nude Morphogenesis/genetics,physiology Peptides/genetics,metabolism Regeneration/genetics,physiology Stem Cells/metabolism
Chemicals
AC133 Antigen Antigens, CD Glycoproteins PROM1 protein, human Peptides Prom1 protein, mouse
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Anderson Lisa H
Boulanger Corinne A
Smith Gilbert H
Carmeliet Peter
Watson Christine J
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25 references, click to expand
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Article Info
Journal
Developmental dynamics : an official publication of the American Association of Anatomists
Abbr.
Dev Dyn
ISSN
1097-0177
DOI
10.1002/dvdy.22539
Published
2011-03-00
Epub
2011-00-11
Pages
674-81
Language
English
Region
United States
NLM ID
9201927
PMCID
PMC3094854
Subset
IM
Grants
Intramural NIH HHS · Z99 CA999999 · United States
External Links
PubMed source DOI: 10.1002/dvdy.22539 PMC Full Text View journal details
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