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题目:: High-throughput cell cycle synchronization using inertial forces in spiral microchannels.
作者:: Lee(Wong Cheng),Bhagat(Ali Asgar S),Huang(Sha),Van Vliet(Krystyn J),Han(Jongyoon),Lim(Chwee Teck)
状态:: 发布时间2011-03-17 , 更新时间 2016-11-25
期刊:: Lab Chip
摘要:: Efficient synchronization and selection of cells at different stages of the cell replication cycle facilitates both fundamental research and development of cell cycle-targeted therapies. Current chemical-based synchronization methods are unfavorable as these can disrupt cell physiology and metabolism. Microfluidic systems developed for physical cell separation offer a potential alternative over conventional cell synchronization approaches. Here we introduce a spiral microfluidic device for cell cycle synchronization, using the combined effects of inertial forces and Dean drag force. By exploiting the relationship between cell diameter and cell cycle (DNA content/ploidy), we have successfully fractionated several asynchronous mammalian cell lines, as well as primary cells comprising bone marrow-derived human mesenchymal stem cells (hMSCs), into enriched subpopulations of G0/G1 (>85%), S, and G2/M phases. This level of cell cycle enrichment is comparable to existing microfluidic systems, but the throughput (∼ 15 × 10(6) cells per h) and viability (∼ 95%) of cells thus synchronized are significantly greater. Further, this platform provides rapid collection of synchronized cells or of diameter-sorted cells post-separation, to enable diverse applications in the study and manipulation of cell proliferation.
语言:: eng
DOI:: 10.1039/c0lc00579g

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