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题目:: Live imaging of dendritic cell-Treg cell interactions.
作者:: Sarris(Milka),Betz(Alexander G)
状态:: 发布时间2011-02-02 , 更新时间 2016-11-22
期刊:: Methods Mol Biol
摘要:: The decision to launch an immune response is made during the interaction of helper T cells and regulatory T cells with dendritic cells. Recognition of antigen leads to formation of immunological synapses at the interface between the cells and to activation of the T cells. The length of interaction between the T cells and dendritic cells influences the functional outcome. We have shown that in the absence of proinflammatory stimuli, regulatory T cells and naive helper T cells interact differently with dendritic cells. Neuropilin-1, which is expressed by most regulatory T cells but not naive helper T cells, promotes prolonged interactions with immature dendritic cells, resulting in higher sensitivity to limiting amounts of antigen. We tracked T cell-dendritic cell interactions in real-time using time-lapse microscopy, assessed synapse formation by immunofluorescence, and measured regulatory T cell activation by dendritic cells using suppression assays.
语言:: eng
DOI:: 10.1007/978-1-61737-979-6_7

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