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PMID: 2126688 Published · ppublish English Comparative Study Journal Article

New norfloxacin resistance gene in Pseudomonas aeruginosa PAO.

Antimicrobial agents and chemotherapy ·Vol. 34 ·No. 9 ·1990-09-00 ·Pages 1757-61

Fukuda H, Hosaka M, Hirai K, Iyobe S

Abstract

A new type of norfloxacin-resistant mutant of Pseudomonas aeruginosa PAO was isolated. This mutant showed cross resistance to imipenem and chloramphenicol and hypersusceptibility to beta-lactam and aminoglycoside antibiotics. The new norfloxacin resistance gene nfxC was mapped near catA (46 min) on the PAO chromosome. Norfloxacin accumulation was decreased in the nfxC mutant; furthermore, the rate of imipenem diffusion through the outer membrane of the nfxC mutant was lower than that of the parent strain. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane proteins showed a decrease of a 46-kilodalton protein and an increase of a 50-kilodalton protein in the nfxC mutant. We conclude the nfxC is a new norfloxacin resistance gene that affects outer membrane permeability to quinolones and other antimicrobial agents.

Related Genes
cfxA cfxB nalA nalB nfxA nfxB nfxC
MeSH Terms
Bacterial Outer Membrane Proteins/genetics Cell Membrane/physiology Chromosome Mapping Drug Resistance, Microbial/genetics Imipenem/pharmacokinetics Microbial Sensitivity Tests Mutation Norfloxacin/pharmacokinetics,pharmacology Permeability Pseudomonas aeruginosa/genetics,metabolism
Chemicals
Bacterial Outer Membrane Proteins Imipenem Norfloxacin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fukuda H
Central Research Laboratories, Kyorin Pharmaceutical Co. Ltd., Tochigi-ken, Japan.
Hosaka M
Hirai K
Iyobe S
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.34.9.1757
Published
1990-09-00
Pages
1757-61
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC171918
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.34.9.1757 PMC Full Text View journal details
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