Home LiteratureArticle Details
PMID: 21264540 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Quantitative analysis of promoter methylation of the EDNRB gene in gastric cancer.

Medical oncology (Northwood, London, England) ·Vol. 29 ·No. 1 ·2012-03-00 ·Pages 107-12

Tao K, Wu C, Wu K, Li W, Han G, Shuai X, Wang G

Abstract

Hypermethylation has been shown in the promoter region of the endothelin receptor B (EDNRB) gene in several human tumors. However, its role in gastric cancer formation is still unclear. In this study, the methylation status of the EDNRB gene in paired gastric cancer tissues and adjacent normal tissues from 96 patients was detected quantitatively using pyrosequencing. The results showed the methylation of promoter of EDNRB gene in gastric cancer (50.42 ± 9.03%) was significantly higher than in adjacent normal tissues (6.47 ± 2.98%) (P < 0.01). Among 96 tumor tissues, promoter hypermethylation of the EDNRB gene was correlated with tumor infiltration (T1: 47.4 ± 7.31% T2:48.2 ± 9.17% T3:52.9 ± 6.48% T4:53.2 ± 10.45%), lymph node metastasis (N0:45.4 ± 6.99% N1:49.0 ± 9.10% N2:52.0 ± 8.40% N3:53.7 ± 9.92%), and distant metastasis (M0:48.9 ± 6.99% M1:53.9 ± 11.98%) (P < 0.05), but it was not associated with other clinicopathological characteristics. In addition, the treatment of the human gastric cancer cell line, SGC-7901, with demethylation agent can restore the expression of EDNRB. Our results suggest that promoter hypermethylation of EDNRB gene is highly prevalent in gastric cancer, which may play a role in the pathogenesis of gastric cancer. Futhermore, hypermethylation of EDNRB gene was remarkably related to infiltration and metastasis of gastric cancer and may attribute to the tumor progression.

MeSH Terms
Base Sequence Biomarkers, Tumor/genetics DNA Methylation/genetics Female Humans Male Middle Aged Molecular Sequence Data Neoplasm Staging Prognosis Promoter Regions, Genetic/genetics Receptors, Endothelin/genetics Reverse Transcriptase Polymerase Chain Reaction Stomach Neoplasms/genetics,pathology
Chemicals
Biomarkers, Tumor Receptors, Endothelin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Tao Kaixiong
Department of Laparoscopic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, Hubei 430022, People's Republic of China.
Wu Chuanqing
Wu Ke
Li Wei
Han Gaoxiong
Shuai Xiaoming
Wang Guobin
References (26)
26 references, click to expand
  1. DNA methylation changes after 5-aza-2'-deoxycytidine therapy in patients with leukemia.
    Cancer Res. 2006 May 15;66(10):5495-503 PMID: 16707479
  2. A missense mutation of the endothelin-B receptor gene in multigenic Hirschsprung's disease.
    Cell. 1994 Dec 30;79(7):1257-66 PMID: 8001158
  3. MAL promoter hypermethylation as a novel prognostic marker in gastric cancer.
    Br J Cancer. 2008 Dec 2;99(11):1802-7 PMID: 19002170
  4. Involvement of endothelin receptors in normal and pathological development of neural crest cells.
    Int J Dev Biol. 2003 Jun;47(5):315-25 PMID: 12895026
  5. Comparison in pathological behaviours & prognosis of gastric cancers from general hospitals between China & Japan.
    Indian J Med Res. 2010 Sep;132:295-302 PMID: 20847376
  6. Relevance of DNA methylation in the management of cancer.
    Lancet Oncol. 2003 Jun;4(6):351-8 PMID: 12788407
  7. The epigenetic progenitor origin of human cancer.
    Nat Rev Genet. 2006 Jan;7(1):21-33 PMID: 16369569
  8. Aberrant methylation of EDNRB and p16 genes in hepatocellular carcinoma (HCC) in Taiwan.
    Oncol Rep. 2006 Feb;15(2):507-11 PMID: 16391877
  9. Alterations in DNA methylation: a fundamental aspect of neoplasia.
    Adv Cancer Res. 1998;72:141-96 PMID: 9338076
  10. Reversal of hypermethylation and reactivation of the RARβ2 gene by natural compounds in cervical cancer cell lines.
    Folia Biol (Praha). 2010;56(5):195-200 PMID: 21138650
  11. Endothelin B receptor gene hypermethylation in prostate adenocarcinoma.
    J Clin Pathol. 2003 Jan;56(1):52-5 PMID: 12499435
  12. Aberrant promoter methylation of EDNRB in lung cancer in Taiwan.
    Oncol Rep. 2006 Jan;15(1):167-72 PMID: 16328051
  13. Detection and interpretation of altered methylation patterns in cancer cells.
    Nat Rev Cancer. 2005 Mar;5(3):223-31 PMID: 15719030
  14. Promoter hypermethylation contributes to frequent inactivation of a putative conditional tumor suppressor gene connective tissue growth factor in ovarian cancer.
    Cancer Res. 2007 Aug 1;67(15):7095-105 PMID: 17671176
  15. A gene hypermethylation profile of human cancer.
    Cancer Res. 2001 Apr 15;61(8):3225-9 PMID: 11309270
  16. Identification of aberrant promoter methylation of EDNRB gene in esophageal squamous cell carcinoma.
    Dis Esophagus. 2009;22(1):55-61 PMID: 18564167
  17. CpG island methylator phenotype in cancer.
    Nat Rev Cancer. 2004 Dec;4(12):988-93 PMID: 15573120
  18. Endothelin-1 promotes cell survival in renal cell carcinoma through the ET(A) receptor.
    Cancer Lett. 2007 Feb 8;246(1-2):139-48 PMID: 16581180
  19. The role of DNA methylation in mammalian epigenetics.
    Science. 2001 Aug 10;293(5532):1068-70 PMID: 11498573
  20. Hypermethylation of the hMLH1 gene promoter in human gastric cancers with microsatellite instability.
    Cancer Res. 1999 Mar 1;59(5):1090-5 PMID: 10070967
  21. Promoter hypermethylation of the EDNRB gene in nasopharyngeal carcinoma.
    Int J Cancer. 2002 Apr 10;98(5):651-5 PMID: 11920632
  22. E-cadherin gene (CDH1) promoter methylation as the second hit in sporadic diffuse gastric carcinoma.
    Oncogene. 2001 Mar 22;20(12):1525-8 PMID: 11313896
  23. Global cancer statistics, 2002.
    CA Cancer J Clin. 2005 Mar-Apr;55(2):74-108 PMID: 15761078
  24. Hypermethylation of CpG islands in primary and metastatic human prostate cancer.
    Cancer Res. 2004 Mar 15;64(6):1975-86 PMID: 15026333
  25. Downregulation of endothelin B receptor in human melanoma cell lines parallel to differentiation genes.
    J Invest Dermatol. 1999 Jun;112(6):925-32 PMID: 10383740
  26. The endothelin receptor B (EDNRB) promoter displays heterogeneous, site specific methylation patterns in normal and tumor cells.
    Hum Mol Genet. 2001 Apr 15;10(9):903-10 PMID: 11309363
Article Info
Journal
Medical oncology (Northwood, London, England)
Abbr.
Med Oncol
ISSN
1559-131X
DOI
10.1007/s12032-010-9805-8
Published
2012-03-00
Epub
2011-00-25
Pages
107-12
Language
English
Region
United States
NLM ID
9435512
Subset
IM
External Links
PubMed source DOI: 10.1007/s12032-010-9805-8 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2