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PMID: 21255729 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Molecular and structural basis of ESCRT-III recruitment to membranes during archaeal cell division.

Molecular cell ·Vol. 41 ·No. 2 ·2011-01-21 ·Pages 186-96

Samson RY, Obita T, Hodgson B, Shaw MK, Chong PL, Williams RL, Bell SD

Abstract

Members of the crenarchaeal kingdom, such as Sulfolobus, divide by binary fission yet lack genes for the otherwise near-ubiquitous tubulin and actin superfamilies of cytoskeletal proteins. Recent work has established that Sulfolobus homologs of the eukaryotic ESCRT-III and Vps4 components of the ESCRT machinery play an important role in Sulfolobus cell division. In eukaryotes, several pathways recruit ESCRT-III proteins to their sites of action. However, the positioning determinants for archaeal ESCRT-III are not known. Here, we identify a protein, CdvA, that is responsible for recruiting Sulfolobus ESCRT-III to membranes. Overexpression of the isolated ESCRT-III domain that interacts with CdvA results in the generation of nucleoid-free cells. Furthermore, CdvA and ESCRT-III synergize to deform archaeal membranes in vitro. The structure of the CdvA/ESCRT-III interface gives insight into the evolution of the more complex and modular eukaryotic ESCRT complex.

MeSH Terms
Archaeal Proteins/analysis,chemistry,physiology Endosomal Sorting Complexes Required for Transport/analysis,chemistry,physiology Gene Expression Regulation, Archaeal Liposomes/metabolism Open Reading Frames Protein Structure, Tertiary Sulfolobus/cytology Transcription, Genetic
Chemicals
Archaeal Proteins Endosomal Sorting Complexes Required for Transport Liposomes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Samson Rachel Y
Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, UK.
Obita Takayuki
Hodgson Ben
Shaw Michael K
Chong Parkson Lee-Gau
Williams Roger L
Bell Stephen D
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Article Info
Journal
Molecular cell
Abbr.
Mol Cell
ISSN
1097-4164
DOI
10.1016/j.molcel.2010.12.018
Published
2011-01-21
Pages
186-96
Language
English
Region
United States
NLM ID
9802571
PMCID
PMC3763469
Subset
IM
Grants
Medical Research Council · MC_U105184308 · United Kingdom
Wellcome Trust · 083639/Z/07/Z · United Kingdom
Wellcome Trust · 086045/Z/08/Z · United Kingdom
Databases
PDB
2XVC
External Links
PubMed source DOI: 10.1016/j.molcel.2010.12.018 PMC Full Text View journal details
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