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PMID: 2124348 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and purification of a Bombyx mori homologue of FTZ-F1.

Nucleic acids research ·Vol. 18 ·No. 24 ·1990-12-25 ·Pages 7229-34

Ueda H, Hirose S

Abstract

Extracts from embryos and from posterior and middle silk glands of the silkworm, Bombyx mori contain a sequence specific DNA binding factor termed BmFTZ-F1. The factor binds to the recognition site of FTZ-F1, a positive regulator of the fushi tarazu gene in Drosophila melanogaster. BmFTZ-F1 and FTZ-F1 share the same methylation interference patterns, the same chromatographic behaviors and similar protease digestion profiles. Anti-FTZ-F1 cross reacts with BmFTZ-F1. These results indicate that BmFTZ-F1 is a B. mori homologue of FTZ-F1. The mobility of the factor-DNA complex formed in the silk gland extract changes depending on the developmental stages. Purification of BmFTZF1 to an almost homogeneous state reveals that the factor is a 73 kd protein.

MeSH Terms

Animals Base Sequence Blotting, Western Bombyx/analysis,anatomy & histology,embryology,genetics Cross Reactions DNA-Binding Proteins/genetics Drosophila Proteins Drosophila melanogaster/genetics Fushi Tarazu Transcription Factors Homeodomain Proteins Insect Hormones/genetics,immunology,isolation & purification,metabolism Methylation Molecular Sequence Data Molecular Weight Sequence Homology, Nucleic Acid Serine Endopeptidases/metabolism

Chemicals

DNA-Binding Proteins Drosophila Proteins Fushi Tarazu Transcription Factors Homeodomain Proteins Insect Hormones ftz protein, Drosophila Serine Endopeptidases glutamyl endopeptidase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ueda H

Genetic Stock Center, National Institute of Genetics, Mishima, Japan.

Hirose S

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/18.24.7229

Published

1990-12-25

Pages

7229-34

Language

English

Region

England

NLM ID

0411011

PMCID

PMC332857

Subset

External Links

PubMed source DOI: 10.1093/nar/18.24.7229 PMC Full Text View journal details

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Identification and purification of a Bombyx mori homologue of FTZ-F1.

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