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PMID: 2122447 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Immunoglobulin switch circular DNA in the mouse infected with Nippostrongylus brasiliensis: evidence for successive class switching from mu to epsilon via gamma 1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 20 ·1990-10-00 ·Pages 7829-33

Yoshida K, Matsuoka M, Usuda S, Mori A, Ishizaka K, Sakano H

Abstract

We have characterized immunoglobulin switch circular DNA in mice infected with the nematode parasite Nippostrongylus brasiliensis. Two kinds of circular DNA were identified in the lymph nodes as excision products of switch recombination of immunoglobulin heavy-chain constant region (CH) genes. One is a recombinant between C mu and C gamma 1 (gamma 1 circle), and the other is a recombinant between C gamma 1 and C epsilon (epsilon circle). In the epsilon circle, a short piece of switch mu (S mu) sequence was inserted between S epsilon and S gamma 1 sequences. The inserted S mu sequence could be a trace of the preceding switch from C mu to C gamma 1. These findings indicate that parasitic infection can induce class switch recombinations in a successive manner, first from C mu to C gamma 1, and then from C gamma 1 to C epsilon.

MeSH Terms
Animals Base Sequence Cells, Cultured Cloning, Molecular DNA, Circular/genetics Female Immunoglobulin Constant Regions/genetics Immunoglobulin Heavy Chains/genetics Immunoglobulin Switch Region/genetics Immunoglobulin lambda-Chains/genetics Immunoglobulin mu-Chains/genetics Lymphocytes/immunology Mice Mice, Inbred BALB C Molecular Sequence Data Nematode Infections/immunology Nippostrongylus/immunology Recombination, Genetic Spleen/immunology
Chemicals
DNA, Circular Immunoglobulin Constant Regions Immunoglobulin Heavy Chains Immunoglobulin lambda-Chains Immunoglobulin mu-Chains
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yoshida K
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Matsuoka M
Usuda S
Mori A
Ishizaka K
Sakano H
References (29)
29 references, click to expand
  1. Antibodies of the secondary response can be expressed without switch recombination in normal mouse B cells.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7189-93 PMID: 6438635
  2. Complete nucleotide sequence of the murine gamma 3 switch region and analysis of switch recombination sites in two gamma 3-expressing hybridomas.
    J Immunol. 1985 Jul;135(1):620-6 PMID: 2987353
  3. DNA sequence of the murine gamma 1 switch segment reveals novel structural elements.
    J Immunol. 1986 Apr 1;136(7):2674-83 PMID: 3081649
  4. B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells.
    J Immunol. 1986 Jun 15;136(12):4538-41 PMID: 3486902
  5. Suppression of in vivo polyclonal IgE responses by monoclonal antibody to the lymphokine B-cell stimulatory factor 1.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9675-8 PMID: 3491987
  6. T cell receptor beta gene sequences in the circular DNA of thymocyte nuclei: direct evidence for intramolecular DNA deletion in V-D-J joining.
    Cell. 1987 May 22;49(4):477-85 PMID: 3502705
  7. Regulation of IgG1 and IgE production by interleukin 4.
    Immunol Rev. 1988 Feb;102:51-75 PMID: 3284816
  8. Patterns and extent of isotype-specificity in the murine H chain switch DNA rearrangement.
    J Immunol. 1990 Jan 1;144(1):363-70 PMID: 2104891
  9. Circular DNA is a product of the immunoglobulin class switch rearrangement.
    Nature. 1990 May 31;345(6274):452-6 PMID: 2111465
  10. Switch circular DNA formed in cytokine-treated mouse splenocytes: evidence for intramolecular DNA deletion in immunoglobulin class switching.
    Cell. 1990 Jul 13;62(1):135-42 PMID: 2114219
  11. Circular DNA is excised by immunoglobulin class switch recombination.
    Cell. 1990 Jul 13;62(1):143-9 PMID: 2364432
  12. Organization of the constant-region gene family of the mouse immunoglobulin heavy chain.
    Cell. 1982 Mar;28(3):499-506 PMID: 6804095
  13. Nucleotide sequences of switch regions of immunoglobulin C epsilon and C gamma genes and their comparison.
    J Biol Chem. 1982 Jul 10;257(13):7322-9 PMID: 6282840
  14. Expression of lymphocyte surface IgE does not require switch recombination.
    Nature. 1982 Jun 24;297(5868):697-9 PMID: 6979716
  15. A deoxyribonuclease which requires nucleoside triphosphate from Micrococcus lysodeikticus. I. Purification and characterization of the deoxyribonuclease activity.
    J Biol Chem. 1970 Feb 25;245(4):767-74 PMID: 4906634
  16. Evidence for control of synthesis of the varible regions of the heavy chains of immunoglobulins G and M by the same gene.
    Proc Natl Acad Sci U S A. 1970 Jun;66(2):337-43 PMID: 4988920
  17. IgE formation in the rat following infection with Nippostrongylus brasiliensis. I. Proliferation and differentiation of IgE-bearing cells.
    Cell Immunol. 1976 Mar 15;22(2):248-61 PMID: 776414
  18. Studies of generation of B-cell diversity in mouse, man, and chicken.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 1:139-45 PMID: 408085
  19. Synthesis of multiple immunoglobulin classes by single lymphocytes.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 1:175-83 PMID: 408086
  20. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  21. An immunoglobulin heavy-chain gene is formed by at least two recombinational events.
    Nature. 1980 Feb 21;283(5749):733-9 PMID: 6766532
  22. Rearrangement of immunoglobulin gamma 1-chain gene and mechanism for heavy-chain class switch.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):919-23 PMID: 6767246
  23. Exon shuffling generates an immunoglobulin heavy chain gene.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):2138-42 PMID: 6769120
  24. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
    Nature. 1980 Aug 14;286(5774):676-83 PMID: 6774258
  25. DNA sequences mediating class switching in alpha-immunoglobulins.
    Science. 1980 Sep 19;209(4463):1360-5 PMID: 6774415
  26. Switch region of immunoglobulin Cmu gene is composed of simple tandem repetitive sequences.
    Nature. 1981 Aug 27;292(5826):845-8 PMID: 6791031
  27. Sequence complexity of circular Epstein-Bar virus DNA in transformed cells.
    J Virol. 1981 Oct;40(1):11-9 PMID: 6270367
  28. T cell-derived B cell differentiation factor(s). Effect on the isotype switch of murine B cells.
    J Exp Med. 1982 Mar 1;155(3):734-48 PMID: 7038025
  29. Induction of an IgE response in mice by Nippostrongylus brasiliensis: characterization of lymphoid cells with intracytoplasmic or surface IgE.
    J Immunol. 1983 Jan;130(1):350-6 PMID: 6600186
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.20.7829
Published
1990-10-00
Pages
7829-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54843
Subset
IM
Grants
NIAID NIH HHS · AI-11202 · United States
NIAID NIH HHS · AI-18790 · United States
Databases
GENBANK
M59871, M59872, M59873, M59874, M59875, M59877, M59878, M59879, M59880, M59881
External Links
PubMed source DOI: 10.1073/pnas.87.20.7829 PMC Full Text View journal details
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