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PMID: 2121828 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The use of diphenylene iodonium, an inhibitor of NADPH oxidase, to investigate the antimicrobial action of human monocyte derived macrophages.

Journal of immunological methods ·Vol. 133 ·No. 2 ·1990-10-19 ·Pages 175-9

Robertson AK, Cross AR, Jones OT, Andrew PW

Abstract

Diphenylene iodonium is an inhibitor of the respiratory burst-generating NADPH oxidase of phagocytes. The effect of this compound on human monocyte-derived macrophages and its usefulness in exploring the antimicrobial mechanisms of phagocytes was examined. 1 microM diphenylene iodonium inhibited hydrogen peroxide production by human macrophages and the activity of these cells against Toxoplasma gondii. At this concentration macrophage degranulation was unaffected.

MeSH Terms

Acetylglucosaminidase/metabolism Animals Cell Degranulation Cell Separation Cells, Cultured Female Humans Hydrogen Peroxide/metabolism Interferon-gamma/pharmacology Macrophages/drug effects,metabolism,microbiology Male Mice NADH, NADPH Oxidoreductases/antagonists & inhibitors NADPH Oxidases Onium Compounds/pharmacology Oxygen/metabolism Phagocytes/drug effects,metabolism,microbiology Recombinant Proteins Tetradecanoylphorbol Acetate/pharmacology Toxoplasma/physiology

Chemicals

Onium Compounds Recombinant Proteins diphenyleneiodonium Interferon-gamma Hydrogen Peroxide NADH, NADPH Oxidoreductases NADPH Oxidases Acetylglucosaminidase Tetradecanoylphorbol Acetate Oxygen

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Robertson A K

Department of Microbiology, University of Leicester, U.K.

Cross A R

Jones O T

Andrew P W

Article Info

Journal

Journal of immunological methods

Abbr.

J Immunol Methods

ISSN

0022-1759

DOI

10.1016/0022-1759(90)90357-2

Published

1990-10-19

Pages

175-9

Language

English

Region

Netherlands

NLM ID

1305440

Subset

External Links

PubMed source DOI: 10.1016/0022-1759(90)90357-2 View journal details

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The use of diphenylene iodonium, an inhibitor of NADPH oxidase, to investigate the antimicrobial action of human monocyte derived macrophages.

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