High level synthesis of immunoglobulins in Chinese hamster ovary cells.

Journal of immunology (Baltimore, Md. : 1950) ·Vol. 145 ·No. 9 ·1990-11-01 ·Pages 3011-6

Wood CR, Dorner AJ, Morris GE, Alderman EM, Wilson D, O'Hara RM, Kaufman RJ

Abstract

The expression of lambda L and microH chain cDNA was examined in Chinese hamster ovary cells. Each cDNA was linked to a different, amplifiable, selectable drug marker gene, and expression was monitored in the presence of increasing concentrations of the selective drugs. Cells were obtained that produced greater than 60 micrograms/10(6) cells/48 h of assembled antibody. This Chinese hamster ovary cell-synthesized IgM was polymeric, and exhibited specific hapten binding and C fixation. The expression strategy employed here may prove useful for the future production of genetically engineered antibodies and other multi-subunit proteins.

MeSH Terms

Animals Antibodies/genetics Antibody Specificity Base Sequence Carrier Proteins/metabolism Cell Line Cloning, Molecular/methods Complement Fixation Tests Cricetinae Cricetulus DNA/genetics Endoplasmic Reticulum Chaperone BiP Genetic Engineering/methods Heat-Shock Proteins Immunoglobulin lambda-Chains/genetics Immunoglobulin mu-Chains/genetics Immunoglobulins/genetics Molecular Chaperones Molecular Sequence Data Nitrophenols/immunology Phenylacetates Recombinant Proteins Transfection

Chemicals

Antibodies Carrier Proteins Endoplasmic Reticulum Chaperone BiP Heat-Shock Proteins Immunoglobulin lambda-Chains Immunoglobulin mu-Chains Immunoglobulins Molecular Chaperones Nitrophenols Phenylacetates Recombinant Proteins 4-hydroxy-5-nitrophenyl acetic acid DNA

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Wood C R

Genetics Institute, Inc., Cambridge, MA 02140.

Dorner A J

Morris G E

Alderman E M

Wilson D

O'Hara R M

Kaufman R J

Article Info

Journal

Journal of immunology (Baltimore, Md. : 1950)

Abbr.

J Immunol

ISSN

0022-1767

Published

1990-11-01

Pages

3011-6

Language

English

Region

United States

NLM ID

2985117R

Subset

External Links

PubMed source View journal details

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High level synthesis of immunoglobulins in Chinese hamster ovary cells.

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