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PMID: 21187903 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Mycobacterium tuberculosis eis regulates autophagy, inflammation, and cell death through redox-dependent signaling.

PLoS pathogens ·Vol. 6 ·No. 12 ·2010-12-16 ·Pages e1001230

Shin DM, Jeon BY, Lee HM, Jin HS, Yuk JM, Song CH, Lee SH, Lee ZW, Cho SN, Kim JM, Friedman RL, Jo EK

Abstract

The "enhanced intracellular survival" (eis) gene of Mycobacterium tuberculosis (Mtb) is involved in the intracellular survival of M. smegmatis. However, its exact effects on host cell function remain elusive. We herein report that Mtb Eis plays essential roles in modulating macrophage autophagy, inflammatory responses, and cell death via a reactive oxygen species (ROS)-dependent pathway. Macrophages infected with an Mtb eis-deletion mutant H37Rv (Mtb-Δeis) displayed markedly increased accumulation of massive autophagic vacuoles and formation of autophagosomes in vitro and in vivo. Infection of macrophages with Mtb-Δeis increased the production of tumor necrosis factor-α and interleukin-6 over the levels produced by infection with wild-type or complemented strains. Elevated ROS generation in macrophages infected with Mtb-Δeis (for which NADPH oxidase and mitochondria were largely responsible) rendered the cells highly sensitive to autophagy activation and cytokine production. Despite considerable activation of autophagy and proinflammatory responses, macrophages infected with Mtb-Δeis underwent caspase-independent cell death. This cell death was significantly inhibited by blockade of autophagy and c-Jun N-terminal kinase-ROS signaling, suggesting that excessive autophagy and oxidative stress are detrimental to cell survival. Finally, artificial over-expression of Eis or pretreatment with recombinant Eis abrogated production of both ROS and proinflammatory cytokines, which depends on the N-acetyltransferase domain of the Eis protein. Collectively, these data indicate that Mtb Eis suppresses host innate immune defenses by modulating autophagy, inflammation, and cell death in a redox-dependent manner.

MeSH Terms
Acetyltransferases Animals Antigens, Bacterial/physiology Autophagy Bacterial Proteins/physiology Cell Death Host-Pathogen Interactions/immunology Immunity, Innate Inflammation Macrophages/metabolism,microbiology Mice Mycobacterium tuberculosis/chemistry,physiology Oxidation-Reduction Oxidative Stress Reactive Oxygen Species/metabolism Signal Transduction/physiology
Chemicals
Antigens, Bacterial Bacterial Proteins Reactive Oxygen Species Acetyltransferases Eis protein, Mycobacterium tuberculosis
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Shin Dong-Min
Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, Korea.
Jeon Bo-Young
Lee Hye-Mi
Jin Hyo Sun
Yuk Jae-Min
Song Chang-Hwa
Lee Sang-Hee
Lee Zee-Won
Cho Sang-Nae
Kim Jin-Man
Friedman Richard L
Jo Eun-Kyeong
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Article Info
Journal
PLoS pathogens
Abbr.
PLoS Pathog
ISSN
1553-7374
Published
2010-12-16
Epub
2010-00-16
Pages
e1001230
Language
English
Region
United States
NLM ID
101238921
PMCID
PMC3002989
Subset
IM
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