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PMID: 21183079 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A tissue-specific atlas of mouse protein phosphorylation and expression.

Cell ·Vol. 143 ·No. 7 ·2010-12-23 ·Pages 1174-89

Huttlin EL, Jedrychowski MP, Elias JE, Goswami T, Rad R, Beausoleil SA, Villén J, Haas W, Sowa ME, Gygi SP

Abstract

Although most tissues in an organism are genetically identical, the biochemistry of each is optimized to fulfill its unique physiological roles, with important consequences for human health and disease. Each tissue's unique physiology requires tightly regulated gene and protein expression coordinated by specialized, phosphorylation-dependent intracellular signaling. To better understand the role of phosphorylation in maintenance of physiological differences among tissues, we performed proteomic and phosphoproteomic characterizations of nine mouse tissues. We identified 12,039 proteins, including 6296 phosphoproteins harboring nearly 36,000 phosphorylation sites. Comparing protein abundances and phosphorylation levels revealed specialized, interconnected phosphorylation networks within each tissue while suggesting that many proteins are regulated by phosphorylation independently of their expression. Our data suggest that the "typical" phosphoprotein is widely expressed yet displays variable, often tissue-specific phosphorylation that tunes protein activity to the specific needs of each tissue. We offer this dataset as an online resource for the biological research community.

MeSH Terms
Animals Gene Expression Profiling Mice/genetics,metabolism Organ Specificity Phosphorylation Protein Kinases/genetics Proteins/metabolism Proteomics
Chemicals
Proteins Protein Kinases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Huttlin Edward L
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Jedrychowski Mark P
Elias Joshua E
Goswami Tapasree
Rad Ramin
Beausoleil Sean A
Villén Judit
Haas Wilhelm
Sowa Mathew E
Gygi Steven P
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2010.12.001
Published
2010-12-23
Pages
1174-89
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC3035969
Subset
IM
Grants
NHGRI NIH HHS · R01 HG003456 · United States
NHGRI NIH HHS · R01 HG003456-06 · United States
NHGRI NIH HHS · HG3456 · United States
External Links
PubMed source DOI: 10.1016/j.cell.2010.12.001 PMC Full Text View journal details
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