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题目:: Real-time electrical impedance-based measurement to distinguish oral cancer cells and non-cancer oral epithelial cells.
作者:: Yang(Liju),Arias(L Renea),Lane(Tonya S),Yancey(Martez D),Mamouni(Jaouad)
状态:: 发布时间2011-01-25 , 更新时间 2016-05-12
期刊:: Anal Bioanal Chem
摘要:: In this study, electrical impedance-based measurements were used to distinguish oral cancer cells and non-cancer oral epithelial cells based on their cellular activities on the microelectrodes in a real-time and label-free manner. CAL 27 and Het-1A cell lines were used as the models of oral cancer cells and non-cancer oral epithelial cells, respectively. Various cellular activities, including cell adhesion, spreading, and proliferation were monitored. We found that both the kinetics of cell spreading and the static impedance-based cell index were feasible to distinguish the two cell types. At each given cell number, CAL 27 cell spreading produced a smaller cell index change rate that was 60-70% of those of Het-1A cells. When cells were fully spread, CAL 27 cells generated a cell index more than four times greater than that of Het-1A cells. Since cell spreading and attachment occurs in the first few hours when they were cultured on the microelectrodes, this impedance-based method could be a rapid label-free and non-invasive approach to distinguish oral cancer cells from non-cancer oral epithelial cells. Cell viability analysis was performed along with the impedance-based analysis. Confocal microscopic imaging analysis showed the difference in cell morphology and the thickness of cell monolayers between the two cell types.
语言:: eng
DOI:: 10.1007/s00216-010-4584-9

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