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PMID: 2116004 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Complete sequence of human vinculin and assignment of the gene to chromosome 10.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 15 ·1990-08-00 ·Pages 5667-71

Weller PA, Ogryzko EP, Corben EB, Zhidkova NI, Patel B, Price GJ, Spurr NK, Koteliansky VE, Critchley DR

Abstract

We have determined the complete sequence of human vinculin, a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Comparison of human and chicken embryo vinculin sequences shows that both proteins contain 1066 amino acids and exhibit a high level of sequence identity (greater than 95%). The region of greatest divergence falls within three 112-amino acid repeats spanning residues 259-589. Interestingly, nematode vinculin lacks one of these central repeats. The regions of human vinculin that are N- and C-terminal to the repeats show 54% and 61% sequence identity, respectively, to nematode vinculin. Southern blots of human genomic DNA hybridized with short vinculin cDNA fragments indicate that there is a single vinculin gene. By using a panel of human-rodent somatic cell hybrids, the human vinculin gene was mapped to chromosome 10q11.2-qter.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chick Embryo Chromosomes, Human, Pair 10 Cytoskeletal Proteins/genetics Genes Humans Molecular Sequence Data Restriction Mapping Sequence Homology, Nucleic Acid Vinculin
Chemicals
Cytoskeletal Proteins Vinculin
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Weller P A
Department of Biochemistry, University of Leicester, United Kingdom.
Ogryzko E P
Corben E B
Zhidkova N I
Patel B
Price G J
Spurr N K
Koteliansky V E
Critchley D R
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.15.5667
Published
1990-08-00
Pages
5667-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54388
Subset
IM
Databases
GENBANK
J04126, M33308, Y00312
External Links
PubMed source DOI: 10.1073/pnas.87.15.5667 PMC Full Text View journal details
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