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PMID: 21153670 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A dual switch in phloem unloading during ovule development in Arabidopsis.

Protoplasma ·Vol. 248 ·No. 1 ·2011-01-00 ·Pages 225-35

Werner D, Gerlitz N, Stadler R

Abstract

Developing flowers are important sinks in Arabidopsis thaliana. Their energy demand is covered by assimilates which are synthesized in source leaves and transported via the vasculature. Assimilates are unloaded either symplastically through plasmodesmata or apoplastically by specific transport proteins. Here we studied the pathway of phloem unloading and post-phloem transport in developing gynoecia. Using phloem-mobile fluorescent tracers, we show that phloem unloading into cells of ovule primordia followed a symplastic pathway. Subsequently, the same tracers could not move out of phloem cells into mature ovules anymore. A further change in the mode of phloem unloading occurred after anthesis. In open flowers as well as in outgrowing siliques, the phloem was again unloaded via the symplast. This observed onset of symplastic phloem unloading was accompanied by a change in frequency of MP17-GFP-labeled plasmodesmata. We could also show that the change in cell-cell connectivity was independent of fertilization and increasing sink demand. The presented results indicate that symplastic connectivity is highly regulated and varies not only between different sink tissues but also between different developmental stages.

MeSH Terms
Arabidopsis/embryology,growth & development,metabolism Flowers/growth & development,metabolism Green Fluorescent Proteins/biosynthesis Ovule/embryology,metabolism Phloem/growth & development,metabolism Plant Proteins/biosynthesis Plasmodesmata/metabolism Pollination Recombinant Fusion Proteins/biosynthesis Seeds/growth & development,metabolism
Chemicals
Plant Proteins Recombinant Fusion Proteins Green Fluorescent Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Werner Dagmar
Lehrstuhl Molekulare Pflanzenphysiologie, Universität Erlangen-Nürnberg, Staudtstrasse 5, 91058, Erlangen, Germany.
Gerlitz Nadja
Stadler Ruth
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Article Info
Journal
Protoplasma
Abbr.
Protoplasma
ISSN
1615-6102
DOI
10.1007/s00709-010-0223-8
Published
2011-01-00
Epub
2010-00-14
Pages
225-35
Language
English
Region
Austria
NLM ID
9806853
Subset
IM
External Links
PubMed source DOI: 10.1007/s00709-010-0223-8 View journal details
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