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PMID: 21111240 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't

Reelin and stk25 have opposing roles in neuronal polarization and dendritic Golgi deployment.

Cell ·Vol. 143 ·No. 5 ·2010-11-24 ·Pages 826-36

Matsuki T, Matthews RT, Cooper JA, van der Brug MP, Cookson MR, Hardy JA, Olson EC, Howell BW

Abstract

The Reelin ligand regulates a Dab1-dependent signaling pathway required for brain lamination and normal dendritogenesis, but the specific mechanisms underlying these actions remain unclear. We find that Stk25, a modifier of Reelin-Dab1 signaling, regulates Golgi morphology and neuronal polarization as part of an LKB1-Stk25-Golgi matrix protein 130 (GM130) signaling pathway. Overexpression of Stk25 induces Golgi condensation and multiple axons, both of which are rescued by Reelin treatment. Reelin stimulation of cultured neurons induces the extension of the Golgi into dendrites, which is suppressed by Stk25 overexpression. In vivo, Reelin and Dab1 are required for the normal extension of the Golgi apparatus into the apical dendrites of hippocampal and neocortical pyramidal neurons. This demonstrates that the balance between Reelin-Dab1 signaling and LKB1-Stk25-GM130 regulates Golgi dispersion, axon specification, and dendrite growth and provides insights into the importance of the Golgi apparatus for cell polarization.

MeSH Terms
Animals Cell Adhesion Molecules, Neuronal/metabolism Cell Line Cell Separation Cells, Cultured Extracellular Matrix Proteins/metabolism Golgi Apparatus/metabolism Hippocampus/metabolism Humans Intracellular Signaling Peptides and Proteins/metabolism Mice Nerve Tissue Proteins/metabolism Neurons/cytology,metabolism Protein Serine-Threonine Kinases/metabolism Rats Reelin Protein Serine Endopeptidases/metabolism
Chemicals
Cell Adhesion Molecules, Neuronal Dab1 protein, mouse Extracellular Matrix Proteins Intracellular Signaling Peptides and Proteins Nerve Tissue Proteins Reelin Protein Reln protein, rat Stk25 protein, mouse Protein Serine-Threonine Kinases RELN protein, human Reln protein, mouse Serine Endopeptidases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Matsuki Tohru
Department of Neuroscience and Physiology, SUNY Upstate Medical University, Syracuse, NY 13210, USA.
Matthews Russell T
Cooper Jonathan A
van der Brug Marcel P
Cookson Mark R
Hardy John A
Olson Eric C
Howell Brian W
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2010.10.029
Published
2010-11-24
Pages
826-36
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC3033572
Subset
IM
Grants
NINDS NIH HHS · NS066071 · United States
Medical Research Council · G0701075 · United Kingdom
Intramural NIH HHS · Z01 NS002987-09 · United States
NINDS NIH HHS · R01 NS066071 · United States
NINDS NIH HHS · R01 NS069660 · United States
NCI NIH HHS · CA41072 · United States
NINDS NIH HHS · NS069660 · United States
NCI NIH HHS · R37 CA041072 · United States
NCI NIH HHS · R01 CA041072 · United States
External Links
PubMed source DOI: 10.1016/j.cell.2010.10.029 PMC Full Text View journal details
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