Abstract
A 22-kilodalton protein purified from the culture supernatant fraction of Pseudomonas aeruginosa (strains PA220 and PAO1) was found to enhance the elastolytic activity of purified P. aeruginosa elastase. N-terminal sequence analysis identified the protein as a fragment of the lasA gene product (P.A. Schad and B.H. Iglewski, J. Bacteriol. 170:2784-2789, 1988). However, comparative analysis with the reported LasA sequence indicated that the purified LasA fragment is longer than the deduced sequence reported. The purified LasA fragment had minimal elastolytic and proteolytic activity and did not enhance the proteolytic activity of purified elastase, yet enhanced the elastolytic activity more than 25-fold. The LasA fragment was found to also enhance the elastolytic activities of thermolysin, human neutrophil elastase, and proteinase K. The results presented here suggest that the LasA protein interacts with the elastin substrate rather than modifying elastase.
MeSH Terms
Bacterial Proteins/isolation & purification,metabolism
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Kinetics
Metalloendopeptidases
Molecular Weight
Pancreatic Elastase/isolation & purification,metabolism
Peptide Hydrolases/metabolism
Pseudomonas aeruginosa/enzymology
Chemicals
Bacterial Proteins
Peptide Hydrolases
Pancreatic Elastase
Metalloendopeptidases
staphylolytic protease
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Peters J E
Department of Microbiology, Ohio State University, Columbus 43210-1292.
Galloway D R
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