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PMID: 21092857 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

NG2+ CNS glial progenitors remain committed to the oligodendrocyte lineage in postnatal life and following neurodegeneration.

Neuron ·Vol. 68 ·No. 4 ·2010-11-18 ·Pages 668-81

Kang SH, Fukaya M, Yang JK, Rothstein JD, Bergles DE

Abstract

The mammalian CNS contains a ubiquitous population of glial progenitors known as NG2+ cells that have the ability to develop into oligodendrocytes and undergo dramatic changes in response to injury and demyelination. Although it has been reported that NG2+ cells are multipotent, their fate in health and disease remains controversial. Here, we generated PDGFαR-CreER transgenic mice and followed their fate in vivo in the developing and adult CNS. These studies revealed that NG2+ cells in the postnatal CNS generate myelinating oligodendrocytes, but not astrocytes or neurons. In regions of neurodegeneration in the spinal cord of ALS mice, NG2+ cells exhibited enhanced proliferation and accelerated differentiation into oligodendrocytes but remained committed to the oligodendrocyte lineage. These results indicate that NG2+ cells in the normal CNS are oligodendrocyte precursors with restricted lineage potential and that cell loss and gliosis are not sufficient to alter the lineage potential of these progenitors.

MeSH Terms
Animals Animals, Newborn Cell Differentiation/genetics Cell Lineage/genetics Cell Proliferation Disease Models, Animal Mice Mice, Transgenic Nerve Degeneration/genetics,metabolism,pathology Neurogenesis/genetics Neuroglia/cytology,metabolism,ultrastructure Oligodendroglia/cytology,pathology,ultrastructure Random Allocation Receptor, Platelet-Derived Growth Factor alpha/genetics,physiology Spinal Cord/cytology,metabolism,ultrastructure Stem Cells/cytology,pathology,ultrastructure
Chemicals
Receptor, Platelet-Derived Growth Factor alpha
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kang Shin H
The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, Baltimore, MD 21205, USA.
Fukaya Masahiro
Yang Jason K
Rothstein Jeffrey D
Bergles Dwight E
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Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
1097-4199
DOI
10.1016/j.neuron.2010.09.009
Published
2010-11-18
Pages
668-81
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC2989827
Subset
IM
Grants
NINDS NIH HHS · NS050274 · United States
NIMH NIH HHS · MH084020 · United States
NINDS NIH HHS · P30 NS050274-05S1 · United States
NINDS NIH HHS · NS051509 · United States
NINDS NIH HHS · P30 NS050274-059003 · United States
NINDS NIH HHS · R01 NS051509-05 · United States
NINDS NIH HHS · P30 NS050274-05 · United States
NINDS NIH HHS · R01 NS051509 · United States
NINDS NIH HHS · P30 NS050274 · United States
NIMH NIH HHS · P50 MH084020 · United States
External Links
PubMed source DOI: 10.1016/j.neuron.2010.09.009 PMC Full Text View journal details
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