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PMID: 21081187 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural Review

Pol II waiting in the starting gates: Regulating the transition from transcription initiation into productive elongation.

Biochimica et biophysica acta ·Vol. 1809 ·No. 1 ·2011-01-00 ·Pages 34-45

Nechaev S, Adelman K

Abstract

Proper regulation of gene expression is essential for the differentiation, development and survival of all cells and organisms. Recent work demonstrates that transcription of many genes, including key developmental and stimulus-responsive genes, is regulated after the initiation step, by pausing of RNA polymerase II during elongation through the promoter-proximal region. Thus, there is great interest in better understanding the events that follow transcription initiation and the ways in which the efficiency of early elongation can be modulated to impact expression of these highly regulated genes. Here we describe our current understanding of the steps involved in the transition from an unstable initially transcribing complex into a highly stable and processive elongation complex. We also discuss the interplay between factors that affect early transcript elongation and the potential physiological consequences for genes that are regulated through transcriptional pausing.

MeSH Terms
DNA/genetics,metabolism Gene Expression Regulation Humans Models, Genetic Nuclear Proteins/metabolism Promoter Regions, Genetic/genetics Protein Binding RNA Polymerase II/metabolism Transcription Factors/metabolism Transcription, Genetic Transcriptional Elongation Factors
Chemicals
NSMF protein, human Nuclear Proteins SUPT5H protein, human Transcription Factors Transcriptional Elongation Factors DNA RNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nechaev Sergei
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
Adelman Karen
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Article Info
Journal
Biochimica et biophysica acta
Abbr.
Biochim Biophys Acta
ISSN
0006-3002
DOI
10.1016/j.bbagrm.2010.11.001
Published
2011-01-00
Epub
2010-00-13
Pages
34-45
Language
English
Region
Netherlands
NLM ID
0217513
PMCID
PMC3021596
Subset
IM
Grants
Intramural NIH HHS · Z01 ES101987 · United States
Intramural NIH HHS · ZIA ES101987-04 · United States
External Links
PubMed source DOI: 10.1016/j.bbagrm.2010.11.001 PMC Full Text
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