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PMID: 21071439 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

ERK1/2 phosphorylate Raptor to promote Ras-dependent activation of mTOR complex 1 (mTORC1).

The Journal of biological chemistry ·Vol. 286 ·No. 1 ·2011-01-07 ·Pages 567-77

Carriere A, Romeo Y, Acosta-Jaquez HA, Moreau J, Bonneil E, Thibault P, Fingar DC, Roux PP

Abstract

The Ras/mitogen-activated protein kinase (MAPK) pathway regulates a variety of cellular processes by activating specific transcriptional and translational programs. Ras/MAPK signaling promotes mRNA translation and protein synthesis, but the exact molecular mechanisms underlying this regulation remain poorly understood. Increasing evidence suggests that the mammalian target of rapamycin (mTOR) plays an essential role in this process. Here, we show that Raptor, an essential scaffolding protein of the mTOR complex 1 (mTORC1), becomes phosphorylated on proline-directed sites following activation of the Ras/MAPK pathway. We found that ERK1 and ERK2 interact with Raptor in cells and mediate its phosphorylation in vivo and in vitro. Using mass spectrometry and phosphospecific antibodies, we found three proline-directed residues within Raptor, Ser(8), Ser(696), and Ser(863), which are directly phosphorylated by ERK1/2. Expression of phosphorylation-deficient alleles of Raptor revealed that phosphorylation of these sites by ERK1/2 normally promotes mTORC1 activity and signaling to downstream substrates, such as 4E-BP1. Our data provide a novel regulatory mechanism by which mitogenic and oncogenic activation of the Ras/MAPK pathway promotes mTOR signaling.

MeSH Terms
Adaptor Proteins, Signal Transducing/chemistry,metabolism Amino Acid Sequence Binding Sites Cell Line Cell Proliferation Humans MAP Kinase Signaling System Mechanistic Target of Rapamycin Complex 1 Mitogen-Activated Protein Kinase 1/metabolism Mitogen-Activated Protein Kinase 3/metabolism Molecular Sequence Data Multiprotein Complexes Phosphorylation Proline/metabolism Proteins/metabolism Regulatory-Associated Protein of mTOR TOR Serine-Threonine Kinases ras Proteins/metabolism
Chemicals
Adaptor Proteins, Signal Transducing Multiprotein Complexes Proteins RPTOR protein, human Regulatory-Associated Protein of mTOR Proline Mechanistic Target of Rapamycin Complex 1 TOR Serine-Threonine Kinases Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 ras Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Carriere Audrey
Department of Pathology, Université de Montréal, Montréal, Québec H3C 3J7, Canada.
Romeo Yves
Acosta-Jaquez Hugo A
Moreau Julie
Bonneil Eric
Thibault Pierre
Fingar Diane C
Roux Philippe P
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
Published
2011-01-07
Epub
2010-00-11
Pages
567-77
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC3013016
Subset
IM
Grants
NIDDK NIH HHS · DK-078135 · United States
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