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PMID: 21038881 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Conservation of molecular interactions stabilizing bovine and mouse rhodopsin.

Biochemistry ·Vol. 49 ·No. 49 ·2010-12-14 ·Pages 10412-20

Kawamura S, Colozo AT, Müller DJ, Park PS

Abstract

Rhodopsin is the light receptor that initiates phototransduction in rod photoreceptor cells. The structure and function of rhodopsin are tightly linked to molecular interactions that stabilize and determine the receptor's functional state. Single-molecule force spectroscopy (SMFS) was used to localize and quantify molecular interactions that structurally stabilize bovine and mouse rhodopsin from native disk membranes of rod photoreceptor cells. The mechanical unfolding of bovine and mouse rhodopsin revealed nine major unfolding intermediates, each intermediate defining a structurally stable segment in the receptor. These stable structural segments had similar localization and occurrence in both bovine and mouse samples. For each structural segment, parameters describing their unfolding energy barrier were determined by dynamic SMFS. No major differences were observed between bovine and mouse rhodopsin, thereby implying that the structures of both rhodopsins are largely stabilized by similar molecular interactions.

MeSH Terms
Amino Acid Sequence Animals Cattle Cell Membrane/chemistry,genetics,metabolism Conserved Sequence Mice Mice, Inbred C57BL Models, Molecular Protein Binding/physiology Protein Stability Protein Unfolding Retinal Rod Photoreceptor Cells/chemistry,metabolism Rhodopsin/chemistry,genetics,metabolism Species Specificity Spectrum Analysis
Chemicals
Rhodopsin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kawamura Shiho
Department of Biosystems Science and Engineering, ETH Zürich, 4058 Basel, Switzerland.
Colozo Alejandro T
Müller Daniel J
Park Paul S-H
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Article Info
Journal
Biochemistry
Abbr.
Biochemistry
ISSN
1520-4995
DOI
10.1021/bi101345x
Published
2010-12-14
Epub
2010-00-11
Pages
10412-20
Language
English
Region
United States
NLM ID
0370623
PMCID
PMC2999666
Subset
IM
Grants
NEI NIH HHS · P30 EY011373 · United States
NEI NIH HHS · P30 EY011373-15 · United States
NEI NIH HHS · P30EY11373 · United States
NEI NIH HHS · R00EY018085 · United States
NEI NIH HHS · R00 EY018085 · United States
NEI NIH HHS · R00 EY018085-05 · United States
External Links
PubMed source DOI: 10.1021/bi101345x PMC Full Text View journal details
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