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PMID: 20978285 Published · ppublish English Journal Article Research Support, N.I.H., Intramural

The RNA transport element of the murine musD retrotransposon requires long-range intramolecular interactions for function.

The Journal of biological chemistry ·Vol. 285 ·No. 53 ·2010-12-31 ·Pages 42097-104

Legiewicz M, Zolotukhin AS, Pilkington GR, Purzycka KJ, Mitchell M, Uranishi H, Bear J, Pavlakis GN, Le Grice SF, Felber BK

Abstract

Retrovirus replication requires specialized transport mechanisms to export genomic mRNA from the nucleus to the cytoplasm of the infected cell. This regulation is mediated by a combination of viral and/or cellular factors that interact with cis-acting RNA export elements linking the viral RNA to the cellular CRM1 or NXF1 nuclear export pathways. Endogenous type D murine LTR retrotransposons (musD) were reported to contain an RNA export element located upstream of the 3'-LTR. Although functionally equivalent, the musD export element, termed the musD transport element, is distinct from the other retroviral RNA export elements, such as the constitutive transport element of simian/Mason-Pfizer monkey retroviruses and the RNA transport element found in rodent intracisternal A-particle LTR retrotransposons. We demonstrate here that the minimal RNA transport element (musD transport element) of musD comprises multiple secondary structure elements that presumably serve as recognition signals for the cellular export machinery. We identified two classes of tertiary interactions, namely kissing loops and a pseudoknot. This work constitutes the first example of an RNA transport element requiring such structural motifs to mediate nuclear export.

MeSH Terms
3' Untranslated Regions Animals Biological Transport HeLa Cells Humans Mice Models, Genetic Mutagenesis, Site-Directed Nucleic Acid Conformation Oligonucleotides, Antisense/genetics Promoter Regions, Genetic RNA/metabolism Retroelements/genetics Retroviridae/genetics Terminal Repeat Sequences
Chemicals
3' Untranslated Regions Oligonucleotides, Antisense Retroelements RNA
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Legiewicz Michal
RT Biochemistry Section, NCI-Frederick, National Institutes of Health, Frederick, Maryland 21702-1201, USA.
Zolotukhin Andrei S
Pilkington Guy R
Purzycka Katarzyna J
Mitchell Michelle
Uranishi Hiroaki
Bear Jenifer
Pavlakis George N
Le Grice Stuart F J
Felber Barbara K
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
DOI
10.1074/jbc.M110.182840
Published
2010-12-31
Epub
2010-00-26
Pages
42097-104
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC3009935
Subset
IM
Grants
Intramural NIH HHS · United States
External Links
PubMed source DOI: 10.1074/jbc.M110.182840 PMC Full Text View journal details
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