Home LiteratureArticle Details
PMID: 20975703 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Calmodulin methyltransferase is an evolutionarily conserved enzyme that trimethylates Lys-115 in calmodulin.

Nature communications ·Vol. 1 ·2010-07-27 ·Pages 43

Magnani R, Dirk LM, Trievel RC, Houtz RL

Abstract

Calmodulin (CaM) is a key mediator of calcium-dependent signalling and is subject to regulatory post-translational modifications, including trimethylation of Lys-115. In this paper, we identify a class I, non-SET domain protein methyltransferase, calmodulin-lysine N-methyltransferase (EC 2.1.1.60). A polypeptide chosen from a fraction enriched in calmodulin methyltransferase activity was trypsinized and analysed by tandem mass spectrometry. The amino-acid sequence obtained identified conserved, homologous proteins of unknown function across a wide range of species, thus implicating a broad role for lysine methylation in calcium-dependent signalling. Encoded by c2orf34, the human homologue is a component of two related multigene deletion syndromes in humans. Human, rat, frog, insect and plant homologues were cloned and Escherichia coli-recombinant proteins catalysed the formation of a trimethyllysyl residue at position 115 in CaM, as verified by product analyses and mass spectrometry.

MeSH Terms
Amino Acid Sequence Animals Calmodulin/metabolism Chromatography, Liquid Chromatography, Thin Layer Electrophoresis, Polyacrylamide Gel Humans Insect Proteins/chemistry,genetics,metabolism Lysine/metabolism Methylation Methyltransferases/chemistry,genetics,metabolism Molecular Sequence Data Plant Proteins/chemistry,genetics,metabolism Protein Processing, Post-Translational Protein Structure, Secondary Rats Sequence Homology, Amino Acid Tandem Mass Spectrometry
Chemicals
Calmodulin Insect Proteins Plant Proteins Methyltransferases calmodulin methyltransferase Lysine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Magnani Roberta
Department of Horticulture, Plant Physiology/Biochemistry/Molecular Biology Program, University of Kentucky, Lexington, Kentucky 40546, USA.
Dirk Lynnette M A
Trievel Raymond C
Houtz Robert L
References (28)
28 references, click to expand
  1. Kinetic manifestation of processivity during multiple methylations catalyzed by SET domain protein methyltransferases.
    Biochemistry. 2007 Mar 27;46(12):3905-15 PMID: 17338551
  2. Chemical synthesis and expression of a calmodulin gene designed for site-specific mutagenesis.
    Biochemistry. 1985 Sep 10;24(19):5090-8 PMID: 3000422
  3. Purification of cyclic 3',5'-nucleotide phosphodiesterase inhibitory protein by affinity chromatography on activator protein coupled to Sepharose.
    Biochemistry. 1978 Jan 10;17(1):120-6 PMID: 201280
  4. Crystallization and preliminary X-ray analysis of two new crystal forms of calmodulin.
    Acta Crystallogr D Biol Crystallogr. 1996 Mar 1;52(Pt 2):411-3 PMID: 15299715
  5. Global distribution of the most prevalent deletions causing hypotonia-cystinuria syndrome.
    Eur J Hum Genet. 2007 Oct;15(10):1029-33 PMID: 17579669
  6. Dynamic regulation of histone lysine methylation by demethylases.
    Mol Cell. 2007 Jan 12;25(1):1-14 PMID: 17218267
  7. Comparison of the NAD Kinase and Myosin Light Chain Kinase Activator Properties of Vertebrate, Higher Plant, and Algal Calmodulins.
    Plant Physiol. 1984 Jul;75(3):796-8 PMID: 16663706
  8. Regulation of histone methylation by demethylimination and demethylation.
    Nat Rev Mol Cell Biol. 2007 Apr;8(4):307-18 PMID: 17342184
  9. Novel eye-specific calmodulin methylation characterized by protein mapping in Drosophila melanogaster.
    Proteomics. 2007 Aug;7(15):2651-8 PMID: 17610210
  10. Protein sequence alignments: a strategy for the hierarchical analysis of residue conservation.
    Comput Appl Biosci. 1993 Dec;9(6):745-56 PMID: 8143162
  11. Calmodulin N-methyltransferase. Partial purification and characterization.
    J Biol Chem. 1986 May 25;261(15):7060-9 PMID: 3700427
  12. Calmodulin N-methyltransferase. Kinetics, mechanism, and inhibitors.
    J Biol Chem. 1996 May 31;271(22):12737-43 PMID: 8662690
  13. Multiple-site trimethylation of ribosomal protein L11 by the PrmA methyltransferase.
    Structure. 2008 Jul;16(7):1059-66 PMID: 18611379
  14. ESyPred3D: Prediction of proteins 3D structures.
    Bioinformatics. 2002 Sep;18(9):1250-6 PMID: 12217917
  15. Analysis of the state of posttranslational calmodulin methylation in developing pea plants.
    Plant Physiol. 1990 Jul;93(3):880-7 PMID: 16667596
  16. The 2p21 deletion syndrome: characterization of the transcription content.
    Genomics. 2005 Aug;86(2):195-211 PMID: 15913950
  17. Chromosomal microdeletions and genes' functions: a cluster of chromosomal microdeletions and the deleted genes' functions.
    Eur J Hum Genet. 2007 Oct;15(10):997-8 PMID: 17625506
  18. JmjC-domain-containing proteins and histone demethylation.
    Nat Rev Genet. 2006 Sep;7(9):715-27 PMID: 16983801
  19. Polypeptide substrate specificity of PsLSMT. A set domain protein methyltransferase.
    J Biol Chem. 2007 Sep 21;282(38):27857-64 PMID: 17635932
  20. Expression of a calmodulin methylation mutant affects the growth and development of transgenic tobacco plants.
    Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8394-8 PMID: 1325656
  21. Regulation of chromatin structure by site-specific histone H3 methyltransferases.
    Nature. 2000 Aug 10;406(6796):593-9 PMID: 10949293
  22. Lysine 2,3-aminomutase. Purification and properties of a pyridoxal phosphate and S-adenosylmethionine-activated enzyme.
    J Biol Chem. 1970 Apr 10;245(7):1778-89 PMID: 5438361
  23. Jalview Version 2--a multiple sequence alignment editor and analysis workbench.
    Bioinformatics. 2009 May 1;25(9):1189-91 PMID: 19151095
  24. Multiple Motif Scanning to identify methyltransferases from the yeast proteome.
    Mol Cell Proteomics. 2009 Jul;8(7):1516-26 PMID: 19351663
  25. How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers.
    Nat Struct Mol Biol. 2007 Nov;14(11):1025-1040 PMID: 17984965
  26. Deletion of C2orf34, PREPL and SLC3A1 causes atypical hypotonia-cystinuria syndrome.
    J Med Genet. 2008 May;45(5):314-8 PMID: 18234729
  27. Multi-system disorder syndromes associated with cystinuria type I.
    Curr Mol Med. 2008 Sep;8(6):544-50 PMID: 18781961
  28. Isolation and kinetic characterization of the calmodulin methyltransferase from sheep brain.
    Biochemistry. 1993 Dec 21;32(50):13974-80 PMID: 8268175
Article Info
Journal
Nature communications
Abbr.
Nat Commun
ISSN
2041-1723
DOI
10.1038/ncomms1044
Published
2010-07-27
Epub
2010-00-27
Pages
43
Language
English
Region
England
NLM ID
101528555
Subset
IM
Grants
NCRR NIH HHS · P20 RR020171 · United States
NIGMS NIH HHS · R01 GM073839 · United States
External Links
PubMed source DOI: 10.1038/ncomms1044 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2