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PMID: 20818391 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Uniform transitions of the general RNA polymerase II transcription complex.

Nature structural & molecular biology ·Vol. 17 ·No. 10 ·2010-10-00 ·Pages 1272-8

Mayer A, Lidschreiber M, Siebert M, Leike K, Söding J, Cramer P

Abstract

We present genome-wide occupancy profiles for RNA polymerase (Pol) II, its phosphorylated forms and transcription factors in proliferating yeast. Pol II exchanges initiation factors for elongation factors during a 5' transition that is completed 150 nucleotides downstream of the transcription start site (TSS). The resulting elongation complex is composed of all the elongation factors and shows high levels of Ser7 and Ser5 phosphorylation on the C-terminal repeat domain (CTD) of Pol II. Ser2 phosphorylation levels increase until 600-1,000 nucleotides downstream of the TSS and do not correlate with recruitment of Spt6 and Pcf11, which bind the Ser2-phosphorylated CTD in vitro. This indicates CTD-independent recruitment mechanisms and CTD masking in vivo. Elongation complexes are productive and disassemble in a two-step 3' transition. Paf1, Spt16 (part of the FACT complex), and the CTD kinases Bur1 and Ctk1 exit upstream of the polyadenylation site, whereas Spt4, Spt5, Spt6, Spn1 (also called Iws1) and Elf1 exit downstream. Transitions are uniform and independent of gene length, type and expression.

MeSH Terms
Cell Division Chromatin/genetics,metabolism Chromatin Immunoprecipitation Chromatography, Affinity Genes, Fungal Genome-Wide Association Study Macromolecular Substances Models, Genetic Phosphorylation Phosphoserine/metabolism Protein Kinases/metabolism Protein Processing, Post-Translational RNA Polymerase II/metabolism RNA, Fungal/metabolism RNA, Messenger/metabolism Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins/metabolism Transcription Factors, TFII/metabolism Transcription, Genetic Transcriptional Elongation Factors/metabolism mRNA Cleavage and Polyadenylation Factors/metabolism
Chemicals
Chromatin Macromolecular Substances PCF11 protein, S cerevisiae RNA, Fungal RNA, Messenger Saccharomyces cerevisiae Proteins Transcription Factors, TFII Transcriptional Elongation Factors mRNA Cleavage and Polyadenylation Factors Phosphoserine Protein Kinases carboxy-terminal domain kinase RNA Polymerase II RPB3 protein, S cerevisiae
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mayer Andreas
Gene Center Munich and Department of Biochemistry, Center for Integrated Protein Science, Ludwig-Maximilians-Universität München, Munich, Germany.
Lidschreiber Michael
Siebert Matthias
Leike Kristin
Söding Johannes
Cramer Patrick
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9985
DOI
10.1038/nsmb.1903
Published
2010-10-00
Epub
2010-00-05
Pages
1272-8
Language
English
Region
United States
NLM ID
101186374
Subset
IM
External Links
PubMed source DOI: 10.1038/nsmb.1903 View journal details
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