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PMID: 20805792 Published · ppublish English Comment Letter Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Exhaustive benchmarking of the yeast two-hybrid system.

Nature methods ·Vol. 7 ·No. 9 ·2010-09-00 ·Pages 667-8; author reply 668

Chen YC, Rajagopala SV, Stellberger T, Uetz P

Abstract

暂无摘要

MeSH Terms

Humans Protein Binding Protein Interaction Mapping/methods,standards Recombinant Proteins/genetics,metabolism Reference Standards Reproducibility of Results Two-Hybrid System Techniques/standards

Chemicals

Recombinant Proteins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Chen Yu-Chi

Rajagopala Seesandra Venkatappa

Stellberger Thorsten

Uetz Peter

References (3)

3 references, click to expand

An experimentally derived confidence score for binary protein-protein interactions.
Nat Methods. 2009 Jan;6(1):91-7 PMID: 19060903
Benchmarking yeast two-hybrid systems using the interactions of bacterial motility proteins.
Proteomics. 2009 Dec;9(23):5296-302 PMID: 19834901
Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome.
Proteome Sci. 2010 Feb 15;8:8 PMID: 20205919

Article Info

Journal

Nature methods

Abbr.

Nat Methods

ISSN

1548-7105

DOI

10.1038/nmeth0910-667

Published

2010-09-00

Pages

667-8; author reply 668

Language

English

Region

United States

NLM ID

101215604

Subset

Grants

NIGMS NIH HHS · R01 GM079710 · United States

NIGMS NIH HHS · R01 GM079710-03 · United States

NIGMS NIH HHS · R01GM79710 · United States

Corrections

CommentOn

PMID: 19060903

External Links

PubMed source DOI: 10.1038/nmeth0910-667 View journal details

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Exhaustive benchmarking of the yeast two-hybrid system.

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