An efficient method of antibody elution for the successive or simultaneous localization of two antigens by immunocytochemistry.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society ·Vol. 26 ·No. 4 ·1978-04-00 ·Pages 322-4

Tramu G, Pillez A, Leonardelli J

Abstract

The method described in this paper is available for removal of antibodies retained by tissue antigen after immunohistochemical staining. Its application to the antehypophysis has allowed the successive or the simultaneous localization of two different hormones.

MeSH Terms

Adrenocorticotropic Hormone/analysis Animals Antibodies Guinea Pigs Immunoenzyme Techniques Immunologic Techniques Luteinizing Hormone/analysis Pituitary Gland/analysis

Chemicals

Antibodies Adrenocorticotropic Hormone Luteinizing Hormone

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Tramu G

Pillez A

Leonardelli J

Article Info

Journal

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Abbr.

J Histochem Cytochem

ISSN

0022-1554

DOI

10.1177/26.4.207771

Published

1978-04-00

Pages

322-4

Language

English

Region

United States

NLM ID

9815334

Subset

External Links

PubMed source DOI: 10.1177/26.4.207771 View journal details

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An efficient method of antibody elution for the successive or simultaneous localization of two antigens by immunocytochemistry.

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