Human rotavirus and its antibody: their coexistence in feces of infants.

Journal of clinical microbiology ·Vol. 7 ·No. 5 ·1978-05-00 ·Pages 405-9

Watanabe H, Gust ID, Holmes IH

Abstract

Rotavirus and its antibody were detected by paper disk solid-phase radioimmunoassay or electron microscopy in feces of infants and young children with acute diarrhea. The fecal specimens in which rotavirus was detectable often contained a high titer of antibodies, which were shown by radioimmunoassay to belong mainly to the immunoglobulin G class. Rotavirus was rarely detected in the specimens containing immunoglobulin A antibodies. By dissociation tests carried out by radioimmunoassay, it was shown that the rotavirus particles in some specimens had the same antibody-binding capacity as did cultured simian rotavirus (SA-11), but antibodies in feces usually had low avidity, probably resulting from enzymatic digestion.

MeSH Terms

Antibodies, Viral/analysis Binding Sites, Antibody Child, Preschool Colostrum/immunology Diarrhea, Infantile/immunology,microbiology Feces/immunology,microbiology Humans Immunoglobulin A/analysis Immunoglobulin G/analysis Infant RNA Viruses/isolation & purification Radioimmunoassay Rotavirus/immunology,isolation & purification

Chemicals

Antibodies, Viral Immunoglobulin A Immunoglobulin G

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Watanabe H

Gust I D

Holmes I H

References (29)

29 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.7.5.405-409.1978

Published

1978-05-00

Pages

405-9

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC274996

Subset

External Links

PubMed source DOI: 10.1128/jcm.7.5.405-409.1978 PMC Full Text View journal details

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Human rotavirus and its antibody: their coexistence in feces of infants.

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