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PMID: 2068095 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Rapid determination of nucleotides that define tRNA(Gly) acceptor identity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 14 ·1991-07-15 ·Pages 6147-51

McClain WH, Foss K, Jenkins RA, Schneider J

Abstract

Expression of the genetic code depends on the recognition of specific tRNAs by the enzymes that aminoacylate them. A computer comparison of tRNA sequences coupled with analysis of mutant nonsense-suppressor tRNAs has revealed the structural features that distinguish the acceptor identity of Escherichia coli tRNA(Gly) from tRNAs that accept phenylalanine, arginine, lysine, and glutamine. On replacement of several nucleotides in the acceptor stem and anticodon of the latter tRNAs with tRNA(Gly)-derived residues, the resulting molecules acquired a tRNA(Gly) identity.

MeSH Terms
Anticodon/genetics Base Sequence Escherichia coli/genetics Glycine-tRNA Ligase/metabolism Molecular Sequence Data Nucleic Acid Conformation RNA, Transfer/genetics RNA, Transfer, Gly/genetics,metabolism Sequence Homology, Nucleic Acid Suppression, Genetic
Chemicals
Anticodon RNA, Transfer, Gly RNA, Transfer Glycine-tRNA Ligase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
McClain W H
Department of Bacteriology, University of Wisconsin, Madison 53706-1567.
Foss K
Jenkins R A
Schneider J
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.14.6147
Published
1991-07-15
Pages
6147-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52039
Subset
IM
Grants
NIGMS NIH HHS · GM42123 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.14.6147 PMC Full Text View journal details
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