Distribution of mitochondrial intron sequences among 21 yeast species.

Current genetics ·Vol. 19 ·No. 2 ·1991-02-00 ·Pages 89-94

Skelly PJ, Maleszka R

Abstract

The mitochondrial and nuclear genomes of 21 yeast species belonging to 12 genera have been tested for the presence of sequences similar to seven S. cerevisiae mitochondrial introns (Se cox1.1,2,3,4,5c, Sc cob.4 and Sc LSU.1) and one K. lactis mitochondrial intron (K1 cox1.2). Some introns, (Sc cox1.4, Sc cob.4, Sc LSU.1 and Kl cox1.2-all group I type), are widely distributed and are found in species with either basidiomycete or ascomycete affinities. This distribution is suggestive of recent sequence transfer between species. The remaining S. cerevisiae introns cross react with an additional species but with no set pattern. Pulsed field gel electrophoretic studies confirm that none of the tested mitochondrial introns cross react with nuclear DNA. These introns are, therefore, mitochondria-specific. Seven strains of K. lactis exhibit striking variability in intron content. In contrast to all mitochondrial introns tested, two introns of nuclear genes (the K. lactis actin gene and the S. cerevisiae RP29B gene) are not detected beyond their source species.

MeSH Terms

DNA, Fungal/genetics DNA, Mitochondrial/genetics Electrophoresis, Agar Gel Introns Kluyveromyces/genetics Nucleic Acid Hybridization Saccharomyces cerevisiae/genetics Species Specificity Yeasts/genetics

Chemicals

DNA, Fungal DNA, Mitochondrial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Skelly P J

Research School of Biological Sciences, Australian National University, Canberra.

Maleszka R

References (24)

24 references, click to expand

Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.
Cell. 1984 May;37(1):67-75 PMID: 6373014
Single base substitution in an intron of oxidase gene compensates splicing defects of the cytochrome b gene.
Nature. 1982 Aug 12;298(5875):628-32 PMID: 6285204
Sequence organization of the mitochondrial genome of yeast--a review.
Gene. 1985;37(1-3):1-17 PMID: 3902568
Elevated levels of petite formation in strains of Saccharomyces cerevisiae restored to respiratory competence. I. Association of both high and moderate frequencies of petite mutant formation with the presence of aberrant mitochondrial DNA.
Genetics. 1985 Nov;111(3):389-402 PMID: 3902563
Group I introns: do they only go home?
Trends Genet. 1989 Jun;5(6):168-72 PMID: 2549662
Molecular basis of the 'box effect', A maturase deficiency leading to the absence of splicing of two introns located in two split genes of yeast mitochondrial DNA.
Eur J Biochem. 1984 Oct 1;144(1):85-93 PMID: 6207024
Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6 PMID: 7001447
Sequence of the Candida albicans gene encoding actin.
Nucleic Acids Res. 1989 Nov 25;17(22):9488 PMID: 2685762
Mobile introns and intron-encoded proteins.
Science. 1989 Dec 1;246(4934):1106-9 PMID: 2479980
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
Rearranged mitochondrial genes in the yeast nuclear genome.
Nature. 1983 Jan 27;301(5898):296-301 PMID: 6296698
Group I introns as mobile genetic elements: facts and mechanistic speculations--a review.
Gene. 1989 Oct 15;82(1):91-114 PMID: 2555264
A high-frequency transformation system for the yeast Kluyveromyces lactis.
Curr Genet. 1982 Nov;6(2):123-8 PMID: 24186478
Assembly of the mitochondrial membrane system. Structure and nucleotide sequence of the gene coding for subunit 1 of yeast cytochrme oxidase.
J Biol Chem. 1980 Dec 25;255(24):11927-41 PMID: 6254986
Mitochondrial class II introns encode proteins related to the reverse transcriptases of retroviruses.
Nature. 1985 Aug 15-21;316(6029):641-3 PMID: 2412125
The mosaic cox1 gene in the mitochondrial genome of Schizosaccharomyces pombe: minimal structural requirements and evolution of group I introns.
Gene. 1986;45(3):289-97 PMID: 3026914
Infectious introns.
Cell. 1989 Feb 10;56(3):323-6 PMID: 2536590
Escape of DNA from mitochondria to the nucleus in Saccharomyces cerevisiae.
Nature. 1990 Jul 26;346(6282):376-9 PMID: 2165219
Isolation of mitochondrial DNA using pulsed field gel electrophoresis.
Nucleic Acids Res. 1989 Sep 25;17(18):7537 PMID: 2678001
Functional domains in introns. RNA processing intermediates in cis- and trans-acting mutants in the penultimate intron of the mitochondrial gene for cytochrome b.
J Biol Chem. 1983 Feb 10;258(3):1991-9 PMID: 6296117
A latent intron-encoded maturase is also an endonuclease needed for intron mobility.
Cell. 1989 Feb 10;56(3):421-30 PMID: 2536592
A petite positive strain of Kluyveromyces lactis has a 300 kb deletion in the rDNA cluster.
Curr Genet. 1989 Dec;16(5-6):429-32 PMID: 2611914
Efficient splicing of two yeast mitochondrial introns controlled by a nuclear-encoded maturase.
EMBO J. 1987 Apr;6(4):1085-91 PMID: 3036491
Nonreciprocal exchange between alleles of the yeast mitochondrial 21S rRNA gene: kinetics and the involvement of a double-strand break.
Cell. 1985 Apr;40(4):887-95 PMID: 3886160

Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00326288

Published

1991-02-00

Pages

89-94

Language

English

Region

United States

NLM ID

8004904

Subset

External Links

PubMed source DOI: 10.1007/BF00326288 View journal details

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Distribution of mitochondrial intron sequences among 21 yeast species.

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