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PMID: 20601958 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Dynamic structure of membrane-anchored Arf*GTP.

Nature structural & molecular biology ·Vol. 17 ·No. 7 ·2010-07-00 ·Pages 876-81

Liu Y, Kahn RA, Prestegard JH

Abstract

ADP ribosylation factors (Arfs) are N-myristoylated GTP/GDP switch proteins that have key regulatory roles in vesicle transport in eukaryotic cells. ARFs execute their roles by anchoring to membrane surfaces, where they interact with other proteins to initiate budding and maturation of transport vesicles. However, existing structures of Arf*GTP are limited to nonmyristoylated and truncated forms with impaired membrane binding. We report a high-resolution NMR structure for full-length myristoylated yeast (Saccharomyces cerevisiae) Arf1 in complex with a membrane mimic. The two-domain structure, in which the myristoylated N-terminal helix is separated from the C-terminal domain by a flexible linker, suggests a level of adaptability in binding modes for the myriad of proteins with which Arf interacts and allows predictions of specific lipid binding sites on some of these proteins.

MeSH Terms
ADP-Ribosylation Factor 1/chemistry,metabolism Amino Acid Sequence Guanosine Triphosphate/chemistry,metabolism Membrane Lipids/metabolism Membranes, Artificial Models, Molecular Molecular Sequence Data Myristic Acids/metabolism Nuclear Magnetic Resonance, Biomolecular Protein Binding Protein Conformation Saccharomyces cerevisiae/chemistry,metabolism
Chemicals
Membrane Lipids Membranes, Artificial Myristic Acids Guanosine Triphosphate ADP-Ribosylation Factor 1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Liu Yizhou
Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA.
Kahn Richard A
Prestegard James H
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9985
DOI
10.1038/nsmb.1853
Published
2010-07-00
Epub
2010-00-04
Pages
876-81
Language
English
Region
United States
NLM ID
101186374
PMCID
PMC2921649
Subset
IM
Grants
NIGMS NIH HHS · R01 GM061268 · United States
NIGMS NIH HHS · R01 GM061268-07 · United States
NIGMS NIH HHS · R01 GM061268-08 · United States
NIGMS NIH HHS · GM61268 · United States
Databases
PDB
2KSQ
External Links
PubMed source DOI: 10.1038/nsmb.1853 PMC Full Text View journal details
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