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PMID: 205674 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Solubilization and characterization of Herpesvirus saimiri-induced membrane antigens.

Journal of virology ·Vol. 25 ·No. 3 ·1978-03-00 ·Pages 852-9

Qaultiere LF, Pearson GR

Abstract

Treatment of Herpesvirus saimiri (HVS)-infected owl monkey cells by limited papain digestion removed the HVS-induced membrane antigen (MA) as determined by membrane immunofluorescence and antibody-dependent lymphocyte cytotoxicity (ADLC). Soluble antigenically active HVSMA was detected by inhibition of ADLC and by the decreased binding of 125I-labeled staphylococcus protein A to HVS-infected cells after absorption of an anti-MA-positive serum with papain extracts. Approximately 38% of the inhibitory activity of the papain extracts was sedimentable at 100,000 X g, indicating that the released MA was heterogeneous in size. Preliminary investigations by gel filtration chromatography identified a major peak of MA with a molecular weight between 20,000 and 50,000.

MeSH Terms
Animals Antigens, Viral/analysis Cell Membrane/immunology Culture Techniques Haplorhini Herpesviridae/immunology Herpesvirus 2, Saimiriine/immunology Molecular Weight Papain Solubility
Chemicals
Antigens, Viral Papain
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Qaultiere L F
Pearson G R
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20 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.25.3.852-859.1978
Published
1978-03-00
Pages
852-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC525979
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.25.3.852-859.1978 PMC Full Text View journal details
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