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PMID: 20562278 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Production of a new thiopeptide antibiotic, TP-1161, by a marine Nocardiopsis species.

Applied and environmental microbiology ·Vol. 76 ·No. 15 ·2010-08-00 ·Pages 4969-76

Engelhardt K, Degnes KF, Kemmler M, Bredholt H, Fjaervik E, Klinkenberg G, Sletta H, Ellingsen TE, Zotchev SB

Abstract

Twenty-seven marine sediment- and sponge-derived actinomycetes with a preference for or dependence on seawater for growth were classified at the genus level using molecular taxonomy. Their potential to produce bioactive secondary metabolites was analyzed by PCR screening for genes involved in polyketide and nonribosomal peptide antibiotic synthesis. Using microwell cultures, conditions for the production of antibacterial and antifungal compounds were identified for 15 of the 27 isolates subjected to this screening. Nine of the 15 active extracts were also active against multiresistant gram-positive bacterial and/or fungal indicator organisms, including vancomycin-resistant Enterococcus faecium and multidrug-resistant Candida albicans. Activity-guided fractionation of fermentation extracts of isolate TFS65-07, showing strong antibacterial activity and classified as a Nocardiopsis species, allowed the identification and purification of the active compound. Structure elucidation revealed this compound to be a new thiopeptide antibiotic with a rare aminoacetone moiety. The in vitro antibacterial activity of this thiopeptide, designated TP-1161, against a panel of bacterial strains was determined.

MeSH Terms
Actinomycetales/classification,genetics,isolation & purification,metabolism Animals Anti-Bacterial Agents/biosynthesis,isolation & purification,pharmacology Antifungal Agents/isolation & purification,metabolism,pharmacology Bacteria/drug effects Candida albicans/drug effects Genes, Fungal Geologic Sediments/microbiology Microbial Sensitivity Tests Molecular Sequence Data Peptides/isolation & purification,pharmacology Porifera/microbiology Sequence Analysis, DNA
Chemicals
Anti-Bacterial Agents Antifungal Agents Peptides
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Engelhardt Kerstin
Norwegian University of Science and Technology, Department of Biotechnology, Sem Saelandsvei 6/8, 7491 Trondheim, Norway.
Degnes Kristin F
Kemmler Michael
Bredholt Harald
Fjaervik Espen
Klinkenberg Geir
Sletta Håvard
Ellingsen Trond E
Zotchev Sergey B
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
DOI
10.1128/AEM.00741-10
Published
2010-08-00
Epub
2010-00-18
Pages
4969-76
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC2916467
Subset
IM
Databases
GENBANK
HM001271, HM001272, HM001273, HM001274, HM001275, HM001276, HM001277, HM001278, HM001279, HM001280, HM001281, HM001282, HM001283, HM001284, HM001285, HM001286, HM001287, HM001288, HM001289, HM001290, HM001291, HM001292, HM001293, HM001294, HM001295, HM001296, HM001297
External Links
PubMed source DOI: 10.1128/AEM.00741-10 PMC Full Text View journal details
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