Production of bovine insulin-like growth factor 2 (bIGF2) in Escherichia coli.

Gene ·Vol. 101 ·No. 2 ·1991-05-30 ·Pages 291-5

Easton AM, Gierse JK, Seetharam R, Klein BK, Kotts CE

Abstract

Bovine insulin-like growth factor 2 (bIGF2) was produced in inclusion bodies in the cytoplasm of Escherichia coli and accumulated at high levels: 20-25% of total Coomassie-stained bacterial protein. The level of accumulation of bIGF2 was affected by the choice of codons in the 5' end of the coding sequence and by a rpoH mutation in the host cells. Purified recombinant bIGF2 had the native N terminus and the same mitogenic activity as that of bIGF2 purified from bovine serum.

Related Genes

bIGF2

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cattle/genetics Cell Division/drug effects Codon Cytoplasm/metabolism Escherichia coli/genetics,metabolism Insulin-Like Growth Factor II/biosynthesis,genetics,pharmacology Molecular Sequence Data Nucleic Acid Conformation Protein Sorting Signals/genetics Recombinant Fusion Proteins/biosynthesis

Chemicals

Codon Protein Sorting Signals Recombinant Fusion Proteins Insulin-Like Growth Factor II

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Easton A M

Department of Biological Sciences, Monsanto Co., Chesterfield, MO 63198.

Gierse J K

Seetharam R

Klein B K

Kotts C E

Article Info

Journal

Gene

Abbr.

Gene

ISSN

0378-1119

DOI

10.1016/0378-1119(91)90426-c

Published

1991-05-30

Pages

291-5

Language

English

Region

Netherlands

NLM ID

7706761

Subset

Databases

GENBANK

M60420, S39290, S39297, S39535, S70115, S70117, S70121, S70125, S70128, S70130

External Links

PubMed source DOI: 10.1016/0378-1119(91)90426-c View journal details

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Production of bovine insulin-like growth factor 2 (bIGF2) in Escherichia coli.

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