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PMID: 2052572 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The TPR-MET oncogenic rearrangement is present and expressed in human gastric carcinoma and precursor lesions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 11 ·1991-06-01 ·Pages 4892-6

Soman NR, Correa P, Ruiz BA, Wogan GN

Abstract

The TPR-MET oncogenic rearrangement was originally observed in an in vitro transformed human osteosarcoma cell line. Recently, we detected the expression of this rearrangement at very low levels in several cell lines derived from human tumors of nonhematopoietic origin using a highly sensitive method based on polymerase chain reaction amplification of the transcript. We report here the results of analysis of TPR-MET expression in cell lines derived from human gastric tumors and 22 biopsy samples of human gastric mucosa showing cancer or precursor lesions. The rearranged RNA was expressed in all four cell lines as well as in biopsy samples from 12 of the 22 patients. Overexpression of TPR-MET RNA in superficial gastritis lesions with hyperplasia of glandular neck cells suggests the possible involvement of this oncogene at an early stage of gastric tumorigenesis. Analysis of gastric biopsy samples for RAS gene mutations showed base substitutions occurring in the codon 12 region of Ki- and Ha-RAS genes in four cases, including two precursor lesions.

Related Genes
TPR-MET
MeSH Terms
Base Sequence Biopsy Cell Line Codon Gastric Mucosa/pathology Gene Rearrangement Humans Molecular Sequence Data Oligonucleotide Probes Oncogenes Osteosarcoma/genetics Polymerase Chain Reaction Polymorphism, Genetic Precancerous Conditions/genetics,pathology RNA, Neoplasm/genetics,isolation & purification Restriction Mapping Stomach Neoplasms/genetics,pathology
Chemicals
Codon Oligonucleotide Probes RNA, Neoplasm
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Soman N R
Division of Toxicology, Whitaker College of Health Sciences and Technology, Cambridge, MA.
Correa P
Ruiz B A
Wogan G N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.11.4892
Published
1991-06-01
Pages
4892-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51773
Subset
IM
Grants
NCI NIH HHS · P01-CA-28842 · United States
NIEHS NIH HHS · P01-ES-00597 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.11.4892 PMC Full Text View journal details
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