Home LiteratureArticle Details
PMID: 20516080 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

myo-Inositol-1-phosphate synthase is required for polar auxin transport and organ development.

The Journal of biological chemistry ·Vol. 285 ·No. 31 ·2010-07-30 ·Pages 24238-47

Chen H, Xiong L

Abstract

myo-Inositol-1-phosphate synthase is a conserved enzyme that catalyzes the first committed and rate-limiting step in inositol biosynthesis. Despite its wide occurrence in all eukaryotes, the role of myo-inositol-1-phosphate synthase and de novo inositol biosynthesis in cell signaling and organism development has been unclear. In this study, we isolated loss-of-function mutants in the Arabidopsis MIPS1 gene from different ecotypes. It was found that all null mips1 mutants are defective in embryogenesis, cotyledon venation patterning, root growth, and root cap development. The mutant roots are also agravitropic and have reduced basipetal auxin transport. mips1 mutants have significantly reduced levels of major phosphatidylinositols and exhibit much slower rates of endocytosis. Treatment with brefeldin A induces slower PIN2 protein aggregation in mips1, indicating altered PIN2 trafficking. Our results demonstrate that MIPS1 is critical for maintaining phosphatidylinositol levels and affects pattern formation in plants likely through regulation of auxin distribution.

MeSH Terms
Arabidopsis/enzymology Cell Membrane/metabolism Endocytosis Exons Gene Expression Profiling Indoleacetic Acids/metabolism Microscopy, Confocal/methods Microscopy, Electron, Scanning/methods Models, Biological Mutation Myo-Inositol-1-Phosphate Synthase/metabolism Phosphatidylinositols/chemistry Plant Roots/metabolism Signal Transduction Tissue Distribution
Chemicals
Indoleacetic Acids Phosphatidylinositols Myo-Inositol-1-Phosphate Synthase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chen Hao
Donald Danforth Plant Science Center, St. Louis, MO 63132, USA.
Xiong Liming
References (55)
55 references, click to expand
  1. Phosphoinositide lipids as signaling molecules: common themes for signal transduction, cytoskeletal regulation, and membrane trafficking.
    Annu Rev Cell Dev Biol. 1998;14:231-64 PMID: 9891784
  2. Inositol hexakisphosphate mobilizes an endomembrane store of calcium in guard cells.
    Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):10091-5 PMID: 12913129
  3. Differential degradation of PIN2 auxin efflux carrier by retromer-dependent vacuolar targeting.
    Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):17812-7 PMID: 19004783
  4. SCARFACE encodes an ARF-GAP that is required for normal auxin efflux and vein patterning in Arabidopsis.
    Plant Cell. 2006 Jun;18(6):1396-411 PMID: 16698946
  5. Genome-wide insertional mutagenesis of Arabidopsis thaliana.
    Science. 2003 Aug 1;301(5633):653-7 PMID: 12893945
  6. Control of inositol biosynthesis in Saccharomyces cerevisiae: properties of a repressible enzyme system in extracts of wild-type (Ino+) cells.
    J Bacteriol. 1976 Apr;126(1):232-42 PMID: 4423
  7. Coordinated polar localization of auxin efflux carrier PIN1 by GNOM ARF GEF.
    Science. 1999 Oct 8;286(5438):316-8 PMID: 10514379
  8. FIERY1 encoding an inositol polyphosphate 1-phosphatase is a negative regulator of abscisic acid and stress signaling in Arabidopsis.
    Genes Dev. 2001 Aug 1;15(15):1971-84 PMID: 11485991
  9. Efflux-dependent auxin gradients establish the apical-basal axis of Arabidopsis.
    Nature. 2003 Nov 13;426(6963):147-53 PMID: 14614497
  10. RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content.
    Planta. 2006 Jun;224(1):125-32 PMID: 16395584
  11. The Arabidopsis thaliana Myo-inositol 1-phosphate synthase1 gene is required for Myo-inositol synthesis and suppression of cell death.
    Plant Cell. 2010 Mar;22(3):888-903 PMID: 20215587
  12. CVP2- and CVL1-mediated phosphoinositide signaling as a regulator of the ARF GAP SFC/VAN3 in establishment of foliar vein patterns.
    Plant J. 2009 Sep;59(6):895-907 PMID: 19473324
  13. A role for inositol hexakisphosphate in the maintenance of basal resistance to plant pathogens.
    Plant J. 2008 Nov;56(4):638-52 PMID: 18643983
  14. The Arabidopsis ATP-binding cassette protein AtMRP5/AtABCC5 is a high affinity inositol hexakisphosphate transporter involved in guard cell signaling and phytate storage.
    J Biol Chem. 2009 Nov 27;284(48):33614-22 PMID: 19797057
  15. Arabidopsis PLDzeta2 regulates vesicle trafficking and is required for auxin response.
    Plant Cell. 2007 Jan;19(1):281-95 PMID: 17259265
  16. Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 function in epidermal cell polarity.
    Plant Cell. 2005 Feb;17(2):525-36 PMID: 15659621
  17. MicroRNA-mediated regulation of stomatal development in Arabidopsis.
    Plant Cell. 2007 Aug;19(8):2417-29 PMID: 17704216
  18. Brefeldin A: deciphering an enigmatic inhibitor of secretion.
    Plant Physiol. 2002 Nov;130(3):1102-8 PMID: 12427977
  19. Localization of myo-inositol-1-phosphate synthase to the endosperm in developing seeds of Arabidopsis.
    J Exp Bot. 2008;59(11):3069-76 PMID: 18603618
  20. VAN3 ARF-GAP-mediated vesicle transport is involved in leaf vascular network formation.
    Development. 2005 Apr;132(7):1699-711 PMID: 15743878
  21. Phosphoinositide regulation of the actin cytoskeleton.
    Annu Rev Physiol. 2003;65:761-89 PMID: 12471164
  22. Localization of the auxin permease AUX1 suggests two functionally distinct hormone transport pathways operate in the Arabidopsis root apex.
    Genes Dev. 2001 Oct 15;15(20):2648-53 PMID: 11641271
  23. The essential nature of sphingolipids in plants as revealed by the functional identification and characterization of the Arabidopsis LCB1 subunit of serine palmitoyltransferase.
    Plant Cell. 2006 Dec;18(12):3576-93 PMID: 17194770
  24. 1 L-myo-Inositol 1-Phosphate Synthase from Arabidopsis thaliana.
    Plant Physiol. 1995 Feb;107(2):613-619 PMID: 12228386
  25. Polar auxin transport: controlling where and how much.
    Trends Plant Sci. 2001 Nov;6(11):535-42 PMID: 11701382
  26. Genetic analysis of osmotic and cold stress signal transduction in Arabidopsis: interactions and convergence of abscisic acid-dependent and abscisic acid-independent pathways.
    Plant Cell. 1997 Nov;9(11):1935-49 PMID: 9401119
  27. Expression of 1L-myoinositol-1-phosphate synthase in organelles.
    Plant Physiol. 2003 Aug;132(4):2240-7 PMID: 12913178
  28. Sterol-dependent endocytosis mediates post-cytokinetic acquisition of PIN2 auxin efflux carrier polarity.
    Nat Cell Biol. 2008 Feb;10(2):237-44 PMID: 18223643
  29. Cotyledon vascular pattern2-mediated inositol (1,4,5) triphosphate signal transduction is essential for closed venation patterns of Arabidopsis foliar organs.
    Plant Cell. 2004 May;16(5):1263-75 PMID: 15100402
  30. Phosphoinositides as regulators in membrane traffic.
    Science. 1996 Mar 15;271(5255):1533-9 PMID: 8599109
  31. Regulation of cell-specific inositol metabolism and transport in plant salinity tolerance.
    Plant Cell. 1998 May;10(5):753-64 PMID: 9596634
  32. Local, efflux-dependent auxin gradients as a common module for plant organ formation.
    Cell. 2003 Nov 26;115(5):591-602 PMID: 14651850
  33. The glycosylphosphatidylinositol (GPI) biosynthetic pathway of bloodstream-form Trypanosoma brucei is dependent on the de novo synthesis of inositol.
    Mol Microbiol. 2006 Jul;61(1):89-105 PMID: 16824097
  34. Embryo-specific silencing of a transporter reduces phytic acid content of maize and soybean seeds.
    Nat Biotechnol. 2007 Aug;25(8):930-7 PMID: 17676037
  35. Gravity-regulated differential auxin transport from columella to lateral root cap cells.
    Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2987-91 PMID: 12594336
  36. Basipetal auxin transport is required for gravitropism in roots of Arabidopsis.
    Plant Physiol. 2000 Feb;122(2):481-90 PMID: 10677441
  37. An "Electronic Fluorescent Pictograph" browser for exploring and analyzing large-scale biological data sets.
    PLoS One. 2007 Aug 08;2(8):e718 PMID: 17684564
  38. Profiling membrane lipids in plant stress responses. Role of phospholipase D alpha in freezing-induced lipid changes in Arabidopsis.
    J Biol Chem. 2002 Aug 30;277(35):31994-2002 PMID: 12077151
  39. GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox.
    Plant Physiol. 2004 Sep;136(1):2621-32 PMID: 15375207
  40. Inositol monophosphatase regulates localization of synaptic components and behavior in the mature nervous system of C. elegans.
    Genes Dev. 2006 Dec 1;20(23):3296-310 PMID: 17158747
  41. myo-Inositol-1,2,3,4,5,6-hexakisphosphate.
    Phytochemistry. 2003 Nov;64(6):1033-43 PMID: 14568069
  42. Phosphoinositides in cell regulation and membrane dynamics.
    Nature. 2006 Oct 12;443(7112):651-7 PMID: 17035995
  43. Complex physiological and molecular processes underlying root gravitropism.
    Plant Mol Biol. 2002 Jun-Jul;49(3-4):305-17 PMID: 12036256
  44. Interaction of calmodulin, a sorting nexin and kinase-associated protein phosphatase with the Brassica oleracea S locus receptor kinase.
    Plant Physiol. 2003 Oct;133(2):919-29 PMID: 14555783
  45. Phosphoinositides in constitutive membrane traffic.
    Physiol Rev. 2004 Jul;84(3):699-730 PMID: 15269334
  46. Laser ablation of root cap cells: implications for models of graviperception.
    Adv Space Res. 1999;24(6):731-8 PMID: 11542616
  47. Phosphoinositide-dependent regulation of VAN3 ARF-GAP localization and activity essential for vascular tissue continuity in plants.
    Development. 2009 May;136(9):1529-38 PMID: 19363154
  48. Crosstalks between myo-inositol metabolism, programmed cell death and basal immunity in Arabidopsis.
    PLoS One. 2009 Oct 08;4(10):e7364 PMID: 19812700
  49. Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency.
    Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3718-23 PMID: 10737809
  50. Light plays an essential role in intracellular distribution of auxin efflux carrier PIN2 in Arabidopsis thaliana.
    PLoS One. 2008 Jan 30;3(1):e1510 PMID: 18231596
  51. The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growth.
    Cell. 2003 Jan 24;112(2):219-30 PMID: 12553910
  52. Mechanism of auxin perception by the TIR1 ubiquitin ligase.
    Nature. 2007 Apr 5;446(7136):640-5 PMID: 17410169
  53. Inositol signaling and plant growth.
    Trends Plant Sci. 2000 Jun;5(6):252-8 PMID: 10838616
  54. Inositol derivatives: evolution and functions.
    Nat Rev Mol Cell Biol. 2008 Feb;9(2):151-61 PMID: 18216771
  55. Dual lipid modification of Arabidopsis Ggamma-subunits is required for efficient plasma membrane targeting.
    Plant Physiol. 2007 Mar;143(3):1119-31 PMID: 17220359
Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
DOI
10.1074/jbc.M110.123661
Published
2010-07-30
Epub
2010-00-01
Pages
24238-47
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC2911297
Subset
IM
External Links
PubMed source DOI: 10.1074/jbc.M110.123661 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2