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PMID: 20498264 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ail binding to fibronectin facilitates Yersinia pestis binding to host cells and Yop delivery.

Infection and immunity ·Vol. 78 ·No. 8 ·2010-08-00 ·Pages 3358-68

Tsang TM, Felek S, Krukonis ES

Abstract

Yersinia pestis, the causative agent of plague, evades host immune responses and rapidly causes disease. The Y. pestis adhesin Ail mediates host cell binding and is critical for Yop delivery. To identify the Ail receptor(s), Ail was purified following overexpression in Escherichia coli. Ail bound specifically to fibronectin, an extracellular matrix protein with the potential to act as a bridge between Ail and host cells. Ail expressed by E. coli also mediated binding to purified fibronectin, and Ail-mediated E. coli adhesion to host cells was dependent on fibronectin. Ail expressed by Y. pestis bound purified fibronectin, as did the Y. pestis adhesin plasminogen activator (Pla). However, a KIM5 Delta ail mutant had decreased binding to host cells, while a KIM5 Delta pla mutant had no significant defect in adhesion. Furthermore, treatment with antifibronectin antibodies decreased Ail-mediated adhesion by KIM5 and the KIM5 Delta pla mutant, indicating that the Ail-fibronectin interaction was important for cell binding. Finally, antifibronectin antibodies inhibited the KIM5-mediated cytotoxicity of host cells in an Ail-dependent fashion. These data indicate that Ail is a key adhesin that mediates binding to host cells through interaction with fibronectin on the surface of host cells, and this interaction is important for Yop delivery by Y. pestis.

MeSH Terms
Bacterial Adhesion Bacterial Outer Membrane Proteins/metabolism Cell Line Cell Survival Escherichia coli/genetics,pathogenicity Fibronectins/metabolism Humans Protein Binding Recombinant Proteins/biosynthesis,isolation & purification Virulence Factors/metabolism Yersinia pestis/pathogenicity
Chemicals
Ail protein, Yersinia pestis Bacterial Outer Membrane Proteins Fibronectins Recombinant Proteins Virulence Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tsang Tiffany M
Department of Microbiology and Immunology, University of Michigan School of Medicine, 1011 N. University, Ann Arbor, MI 48109-1078, USA.
Felek Suleyman
Krukonis Eric S
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
1098-5522
DOI
10.1128/IAI.00238-10
Published
2010-08-00
Epub
2010-00-24
Pages
3358-68
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC2916272
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.00238-10 PMC Full Text View journal details
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