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PMID: 20435906 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cucumber mosaic virus movement protein severs actin filaments to increase the plasmodesmal size exclusion limit in tobacco.

The Plant cell ·Vol. 22 ·No. 4 ·2010-04-00 ·Pages 1373-87

Su S, Liu Z, Chen C, Zhang Y, Wang X, Zhu L, Miao L, Wang XC, Yuan M

Abstract

Plant viral movement proteins (MPs) enable viruses to pass through cell walls by increasing the size exclusion limit (SEL) of plasmodesmata (PD). Here, we report that the ability of Cucumber mosaic virus (CMV) MP to increase the SEL of the PD could be inhibited by treatment with the actin filament (F-actin)-stabilizing agent phalloidin but not by treatment with the F-actin-destabilizing agent latrunculin A. In vitro studies showed that CMV MP bound globular and F-actin, inhibited actin polymerization, severed F-actin, and participated in plus end capping of F-actin. Analyses of two CMV MP mutants, one with and one without F-actin severing activities, demonstrated that the F-actin severing ability was required to increase the PD SEL. Furthermore, the Tobacco mosaic virus MP also exhibited F-actin severing activity, and its ability to increase the PD SEL was inhibited by treatment with phalloidin. Our data provide evidence to support the hypothesis that F-actin severing is required for MP-induced increase in the SEL of PD. This may have broad implications in the study of the mechanisms of actin dynamics that regulate cell-to-cell transport of viral and endogenous proteins.

MeSH Terms
Actin Cytoskeleton/metabolism Actins/metabolism Cucumovirus/physiology Phalloidine/pharmacology Plant Diseases/virology Plant Viral Movement Proteins/metabolism Plasmodesmata/metabolism Tobacco/virology
Chemicals
Actins Plant Viral Movement Proteins Phalloidine
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Su Shengzhong
State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Liu Zhaohui
Chen Cheng
Zhang Yan
Wang Xu
Zhu Lei
Miao Long
Wang Xue-Chen
Yuan Ming
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.108.064212
Published
2010-04-00
Epub
2010-00-30
Pages
1373-87
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2879750
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.108.064212 PMC Full Text View journal details
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