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PMID: 20385821 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Hybrid PET-optical imaging using targeted probes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 107 ·No. 17 ·2010-04-27 ·Pages 7910-5

Nahrendorf M, Keliher E, Marinelli B, Waterman P, Feruglio PF, Fexon L, Pivovarov M, Swirski FK, Pittet MJ, Vinegoni C, Weissleder R

Abstract

Fusion imaging of radionuclide-based molecular (PET) and structural data [x-ray computed tomography (CT)] has been firmly established. Here we show that optical measurements [fluorescence-mediated tomography (FMT)] show exquisite congruence to radionuclide measurements and that information can be seamlessly integrated and visualized. Using biocompatible nanoparticles as a generic platform (containing a (18)F isotope and a far red fluorochrome), we show good correlations between FMT and PET in probe concentration (r(2) > 0.99) and spatial signal distribution (r(2) > 0.85). Using a mouse model of cancer and different imaging probes to measure tumoral proteases, macrophage content and integrin expression simultaneously, we demonstrate the distinct tumoral locations of probes in multiple channels in vivo. The findings also suggest that FMT can serve as a surrogate modality for the screening and development of radionuclide-based imaging agents.

MeSH Terms
Animals Flow Cytometry Fluorescent Dyes Image Processing, Computer-Assisted/methods Mice Mice, Inbred C57BL Nanoparticles Neoplasms/diagnosis Positron-Emission Tomography/methods Tomography, X-Ray Computed/methods
Chemicals
Fluorescent Dyes
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Nahrendorf Matthias
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. MNahrendorf@mgh.harvard.edu
Keliher Edmund
Marinelli Brett
Waterman Peter
Feruglio Paolo Fumene
Fexon Lioubov
Pivovarov Misha
Swirski Filip K
Pittet Mikael J
Vinegoni Claudio
Weissleder Ralph
References (37)
37 references, click to expand
  1. Detection of colonic dysplasia in vivo using a targeted heptapeptide and confocal microendoscopy.
    Nat Med. 2008 Apr;14(4):454-8 PMID: 18345013
  2. A comparison of PET imaging characteristics of various copper radioisotopes.
    Eur J Nucl Med Mol Imaging. 2005 Dec;32(12):1473-80 PMID: 16258764
  3. Facile, efficient approach to accomplish tunable chemistries and variable biodistributions for shell cross-linked nanoparticles.
    Biomacromolecules. 2008 Jul;9(7):1997-2006 PMID: 18510359
  4. Multispectral fluorescence imaging.
    J Nucl Med. 2009 Oct;50(10):1563-6 PMID: 19759119
  5. Imaging of differential protease expression in breast cancers for detection of aggressive tumor phenotypes.
    Radiology. 2002 Mar;222(3):814-8 PMID: 11867806
  6. Design considerations for tumour-targeted nanoparticles.
    Nat Nanotechnol. 2010 Jan;5(1):42-7 PMID: 19893516
  7. Detection of invasive colon cancer using a novel, targeted, library-derived fluorescent peptide.
    Cancer Res. 2004 Sep 1;64(17):6247-51 PMID: 15342411
  8. Fusion of contrast-enhanced breast MR and mammographic imaging data.
    Med Image Anal. 2003 Sep;7(3):311-40 PMID: 12946471
  9. Monocyte subset dynamics in human atherosclerosis can be profiled with magnetic nano-sensors.
    PLoS One. 2009 May 22;4(5):e5663 PMID: 19461894
  10. Imaging transgenic animals.
    Annu Rev Biomed Eng. 1999;1:611-48 PMID: 11701502
  11. Hybrid in vivo FMT-CT imaging of protease activity in atherosclerosis with customized nanosensors.
    Arterioscler Thromb Vasc Biol. 2009 Oct;29(10):1444-51 PMID: 19608968
  12. Behavior of endogenous tumor-associated macrophages assessed in vivo using a functionalized nanoparticle.
    Neoplasia. 2009 May;11(5):459-68, 2 p following 468 PMID: 19412430
  13. 18F labeled nanoparticles for in vivo PET-CT imaging.
    Bioconjug Chem. 2009 Feb;20(2):397-401 PMID: 19138113
  14. The FLARE intraoperative near-infrared fluorescence imaging system: a first-in-human clinical trial in breast cancer sentinel lymph node mapping.
    Ann Surg Oncol. 2009 Oct;16(10):2943-52 PMID: 19582506
  15. Biomedical imaging research opportunities workshop IV: a white paper.
    Med Phys. 2007 Feb;34(2):673-9 PMID: 17388185
  16. Hybrid PET/CT is greater than the sum of its parts.
    J Nucl Cardiol. 2008 Jan-Feb;15(1):118-22 PMID: 18242488
  17. The advantages of nanoparticles for PET.
    J Nucl Med. 2009 Nov;50(11):1743-6 PMID: 19837751
  18. Nanoparticle PET-CT imaging of macrophages in inflammatory atherosclerosis.
    Circulation. 2008 Jan 22;117(3):379-87 PMID: 18158358
  19. Multimodality imaging of tumor xenografts and metastases in mice with combined small-animal PET, small-animal CT, and bioluminescence imaging.
    J Nucl Med. 2007 Feb;48(2):295-303 PMID: 17268028
  20. A methodology for registration of a histological slide and in vivo MRI volume based on optimizing mutual information.
    Mol Imaging. 2006 Jan-Mar;5(1):16-23 PMID: 16779966
  21. Intraoperative identification of sentinel lymph nodes by near-infrared fluorescence imaging in patients with breast cancer.
    Am J Surg. 2008 Jun;195(6):850-3 PMID: 18353274
  22. Fluorogenic 1,3-dipolar cycloaddition within the hydrophobic core of a shell cross-linked nanoparticle.
    Chemistry. 2006 Sep 6;12(26):6776-86 PMID: 16800009
  23. Noninvasive vascular cell adhesion molecule-1 imaging identifies inflammatory activation of cells in atherosclerosis.
    Circulation. 2006 Oct 3;114(14):1504-11 PMID: 17000904
  24. Diffuse optical tomography with a priori anatomical information.
    Phys Med Biol. 2005 Jun 21;50(12):2837-58 PMID: 15930606
  25. Imaging in the era of molecular oncology.
    Nature. 2008 Apr 3;452(7187):580-9 PMID: 18385732
  26. Standardized methods for the production of high specific-activity zirconium-89.
    Nucl Med Biol. 2009 Oct;36(7):729-39 PMID: 19720285
  27. Cell-specific targeting of nanoparticles by multivalent attachment of small molecules.
    Nat Biotechnol. 2005 Nov;23(11):1418-23 PMID: 16244656
  28. Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci.
    Cancer Res. 2007 Jul 15;67(14):6882-8 PMID: 17638899
  29. Partial colocalization of glucocorticoid and mineralocorticoid receptors in discrete compartments in nuclei of rat hippocampus neurons.
    J Cell Sci. 1996 Apr;109 ( Pt 4):787-92 PMID: 8718670
  30. Biodegradable dendritic positron-emitting nanoprobes for the noninvasive imaging of angiogenesis.
    Proc Natl Acad Sci U S A. 2009 Jan 20;106(3):685-90 PMID: 19129498
  31. Identification of splenic reservoir monocytes and their deployment to inflammatory sites.
    Science. 2009 Jul 31;325(5940):612-6 PMID: 19644120
  32. MRI-guided diffuse optical spectroscopy of malignant and benign breast lesions.
    Neoplasia. 2002 Jul-Aug;4(4):347-54 PMID: 12082551
  33. Cancer drug development with the help of radiopharmaceuticals: academic experience.
    Curr Pharm Des. 2009;15(9):950-6 PMID: 19275659
  34. A multimodal nanoparticle for preoperative magnetic resonance imaging and intraoperative optical brain tumor delineation.
    Cancer Res. 2003 Dec 1;63(23):8122-5 PMID: 14678964
  35. Synergistic effects of light-emitting probes and peptides for targeting and monitoring integrin expression.
    Proc Natl Acad Sci U S A. 2005 May 31;102(22):7976-81 PMID: 15911748
  36. Coating thickness of magnetic iron oxide nanoparticles affects R2 relaxivity.
    J Magn Reson Imaging. 2007 Dec;26(6):1634-41 PMID: 17968941
  37. Segmentation of bionano images for understanding cell dynamics.
    Conf Proc IEEE Eng Med Biol Soc. 2004;2004:1759-62 PMID: 17272047
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0915163107
Email
mnahrendorf@mgh.harvard.edu
Published
2010-04-27
Epub
2010-00-12
Pages
7910-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2867879
Subset
IM
Grants
NHLBI NIH HHS · R01 HL095612 · United States
NCI NIH HHS · R24 CA092782 · United States
NHLBI NIH HHS · R01HL096576 · United States
NCI NIH HHS · P50 CA086355 · United States
NHLBI NIH HHS · UO1 HL08073 · United States
NCI NIH HHS · P50-CA86355 · United States
NHLBI NIH HHS · R01 HL095629 · United States
NCI NIH HHS · U54 CA126515 · United States
NCI NIH HHS · R24 CA92782 · United States
NIBIB NIH HHS · R01 EB006432 · United States
NHLBI NIH HHS · R01 HL096576 · United States
Corrections
CommentIn
PMID: 20870051
External Links
PubMed source DOI: 10.1073/pnas.0915163107 PMC Full Text View journal details
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