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PMID: 2037688 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

DNA fingerprinting by pulsed field gel electrophoresis and ribotyping to distinguish Pseudomonas cepacia isolates from a nosocomial outbreak.

Journal of clinical microbiology ·Vol. 29 ·No. 3 ·1991-03-00 ·Pages 648-9

Anderson DJ, Kuhns JS, Vasil ML, Gerding DN, Janoff EN

Abstract

We typed 40 isolates of Pseudomonas cepacia obtained from patients involved in a single outbreak using pulsed field gel electrophoresis and ribotyping. All isolates from the majority of the patients, 16 of 18 (89%), were included in a single group. These typing methods should aid in the clarification of the epidemiology of infection with P. cepacia.

MeSH Terms

Bacterial Typing Techniques Cross Infection/epidemiology,microbiology DNA Fingerprinting/methods DNA, Bacterial/genetics,isolation & purification Electrophoresis, Agar Gel Evaluation Studies as Topic Humans Pseudomonas/classification,genetics,isolation & purification Pseudomonas Infections/epidemiology,microbiology

Chemicals

DNA, Bacterial

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Anderson D J

Department of Medicine, University of Minnesota School of Medicine, Minneapolis 55455.

Kuhns J S

Vasil M L

Gerding D N

Janoff E N

References (8)

8 references, click to expand

Detection of specific sequences among DNA fragments separated by gel electrophoresis.
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Pseudomonas species bacteremia caused by contaminated normal human serum albumin.
J Infect Dis. 1977 May;135(5):729-35 PMID: 404366
Pseudomonas cepacia infection in cystic fibrosis: an emerging problem.
J Pediatr. 1984 Feb;104(2):206-10 PMID: 6420530
Molecular analysis of hemolytic and phospholipase C activities of Pseudomonas cepacia.
Infect Immun. 1990 Dec;58(12):4020-9 PMID: 2254027
A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA.
J Infect Dis. 1988 Feb;157(2):280-6 PMID: 2447202
Ribotype analysis of Pseudomonas cepacia from cystic fibrosis treatment centers.
J Pediatr. 1988 Nov;113(5):859-62 PMID: 2903229
Pseudomonas cepacia typing systems: collaborative study to assess their potential in epidemiologic investigations.
Rev Infect Dis. 1989 Jul-Aug;11(4):600-7 PMID: 2772466
Pseudomonas cepacia: an emerging pathogen in chronic granulomatous disease.
J Pediatr. 1986 Jun;108(6):940-2 PMID: 3486968

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.29.3.648-649.1991

Published

1991-03-00

Pages

648-9

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC269838

Subset

Grants

NIAID NIH HHS · AI-15946 · United States

External Links

PubMed source DOI: 10.1128/jcm.29.3.648-649.1991 PMC Full Text View journal details

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DNA fingerprinting by pulsed field gel electrophoresis and ribotyping to distinguish Pseudomonas cepacia isolates from a nosocomial outbreak.

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