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PMID: 20363788
Published · ppublish
English
Journal Article
Research Support, Non-U.S. Gov't
Effects of plant genotype and growth stage on the betaproteobacterial communities associated with different potato cultivars in two fields.
Abstract
Bacterial communities in the rhizosphere are dynamic and susceptible to changes in plant conditions. Among the bacteria, the betaproteobacteria play key roles in nutrient cycling and plant growth promotion, and hence the dynamics of their community structures in the rhizosphere should be investigated. Here, the effects of plant cultivar, growth stage, and soil type on the communities associated with potato cultivars Aveka, Aventra, Karnico, Modena, Premiere, and Désirée were assessed for two different fields containing sandy soil with either a high or low organic compound content. Thus, bacterial and betaproteobacterial PCR-denaturing gradient gel electrophoresis analyses were performed to analyze the effects of plant cultivar and growth on the rhizosphere community structure. The analyses showed that in both fields all cultivars had a rhizosphere effect on the total bacterial and betaproteobacterial communities. In addition, the plant growth stage strongly affected the betaproteobacterial communities in both fields. Moreover, the community structures were affected by cultivar, and cultivars differed in physiology, as reflected in their growth rates, root development, and estimated tuber starch contents. Analyses of betaproteobacterial clone libraries constructed for two selected cultivars (one cultivar that produced low-starch-content tubers and one cultivar that produced high-starch-content tubers), as well as bulk soil, revealed that the rhizospheres of the two cultivars selected for specific bacteria, including plant-growth-promoting bacteria, such as Variovorax and Achromobacter spp. In addition, quantitative PCR-based quantification of the Variovorax paradoxus-specific functional gene asfA (involved in desulfonation) indicated that there were clear potato rhizosphere effects on the abundance of this gene. Interestingly, both cultivar type and plant growth stage affected the community under some circumstances.
MeSH Terms
Betaproteobacteria/classification,genetics,isolation & purification
Biodiversity
Cluster Analysis
DNA Fingerprinting
DNA, Bacterial/chemistry,genetics
DNA, Ribosomal/chemistry,genetics
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Nucleic Acid Denaturation
Phylogeny
Plant Roots/microbiology
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Soil Microbiology
Solanum tuberosum/genetics,growth & development,microbiology
Chemicals
DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S
References (35)
35 references, click to expand
-
Rhizosphere bacteria affected by transgenic potatoes with antibacterial activities compared with the effects of soil, wild-type potatoes, vegetation stage and pathogen exposure.FEMS Microbiol Ecol. 2006 May;56(2):219-35 PMID: 16629752
-
A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences.J Mol Evol. 1980 Dec;16(2):111-20 PMID: 7463489
-
How to get more out of molecular fingerprints: practical tools for microbial ecology.Environ Microbiol. 2008 Jun;10(6):1571-81 PMID: 18331337
-
Effect of above-ground plant species on soil microbial community structure and its impact on suppression of Rhizoctonia solani AG3.Environ Microbiol. 2006 Feb;8(2):233-46 PMID: 16423012
-
Effect of transferring 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase genes into Pseudomonas fluorescens strain CHA0 and its gacA derivative CHA96 on their growth-promoting and disease-suppressive capacities.Can J Microbiol. 2000 Oct;46(10):898-907 PMID: 11068676
-
Plant growth-promoting bacteria confer resistance in tomato plants to salt stress.Plant Physiol Biochem. 2004 Jun;42(6):565-72 PMID: 15246071
-
Effects of T4 lysozyme release from transgenic potato roots on bacterial rhizosphere communities are negligible relative to natural factors.Appl Environ Microbiol. 2002 Mar;68(3):1325-35 PMID: 11872484
-
Microdiversity of Burkholderiales associated with mycorrhizal and nonmycorrhizal roots of Medicago truncatula.FEMS Microbiol Ecol. 2008 Aug;65(2):180-92 PMID: 18507681
-
Analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomal DNA fragments.Appl Environ Microbiol. 1997 Apr;63(4):1489-97 PMID: 9097446
-
Effects of plant genotype and growth stage on the structure of bacterial communities associated with potato (Solanum tuberosum L.).FEMS Microbiol Ecol. 2008 May;64(2):283-96 PMID: 18355298
-
Legume symbiotic nitrogen fixation by beta-proteobacteria is widespread in nature.J Bacteriol. 2003 Dec;185(24):7266-72 PMID: 14645288
-
Effect of Sphingobium yanoikuyae B1 inoculation on bacterial community dynamics and polycyclic aromatic hydrocarbon degradation in aged and freshly PAH-contaminated soils.Environ Pollut. 2006 Nov;144(1):228-37 PMID: 16524654
-
Variation of microbial communities in soil, rhizosphere, and rhizoplane in response to crop species, soil type, and crop development.Appl Environ Microbiol. 2001 Dec;67(12):5849-54 PMID: 11722945
-
Soil Type and Maize Cultivar Affect the Genetic Diversity of Maize Root-Associated Burkholderia cepacia Populations.Microb Ecol. 1999 Oct;38(3):273-284 PMID: 10541789
-
Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness.Appl Environ Microbiol. 2005 Mar;71(3):1501-6 PMID: 15746353
-
Changes in developing plant microbial community structure as affected by contaminated water.FEMS Microbiol Ecol. 2004 May 1;48(2):239-48 PMID: 19712407
-
Influence of Arabidopsis thaliana accessions on rhizobacterial communities and natural variation in root exudates.J Exp Bot. 2009;60(6):1729-42 PMID: 19342429
-
Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients.Appl Environ Microbiol. 1997 Aug;63(8):3233-41 PMID: 9251210
-
Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis.J Microbiol Methods. 1999 Oct;38(1-2):1-15 PMID: 10520580
-
PCR bias in ecological analysis: a case study for quantitative Taq nuclease assays in analyses of microbial communities.Appl Environ Microbiol. 2000 Nov;66(11):4945-53 PMID: 11055948
-
Abundance, composition, diversity and novelty of soil Proteobacteria.ISME J. 2009 Aug;3(8):992-1000 PMID: 19404326
-
The role of Variovorax and other Comamonadaceae in sulfur transformations by microbial wheat rhizosphere communities exposed to different sulfur fertilization regimes.Environ Microbiol. 2008 Jun;10(6):1486-500 PMID: 18279342
-
Seasonal changes in the rhizosphere microbial communities associated with field-grown genetically modified canola (Brassica napus).Appl Environ Microbiol. 2003 Dec;69(12):7310-8 PMID: 14660380
-
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.Appl Environ Microbiol. 1993 Mar;59(3):695-700 PMID: 7683183
-
The role of soil microbes in plant sulphur nutrition.J Exp Bot. 2004 Aug;55(404):1939-45 PMID: 15181108
-
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.Mol Biol Evol. 2007 Aug;24(8):1596-9 PMID: 17488738
-
A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop.Nat Biotechnol. 2003 Apr;21(4):439-42 PMID: 12627169
-
Analysis of the dynamics of bacterial communities in the rhizosphere of the chrysanthemum via denaturing gradient gel electrophoresis and substrate utilization patternsAppl Environ Microbiol. 1998 Dec;64(12):4950-7 PMID: 9835588
-
Desulfurization of aromatic sulfonates by rhizosphere bacteria: high diversity of the asfA gene.Environ Microbiol. 2007 Feb;9(2):535-45 PMID: 17222151
-
Identification of bacterial groups preferentially associated with mycorrhizal roots of Medicago truncatula.Appl Environ Microbiol. 2007 Feb;73(3):913-21 PMID: 17142371
-
Microbial diversity in soil: selection microbial populations by plant and soil type and implications for disease suppressiveness.Annu Rev Phytopathol. 2004;42:243-70 PMID: 15283667
-
The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data.Nucleic Acids Res. 2007 Jan;35(Database issue):D169-72 PMID: 17090583
-
Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-beneficial properties.Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1187-1192 PMID: 15879253
-
Burkholderia tropica sp. nov., a novel nitrogen-fixing, plant-associated bacterium.Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2155-2162 PMID: 15545451
-
Quantitative comparisons of 16S rRNA gene sequence libraries from environmental samples.Appl Environ Microbiol. 2001 Sep;67(9):4374-6 PMID: 11526051
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
Published
2010-06-00
Epub
2010-00-02
Pages
3675-84
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC2876460
Subset
IM
Databases
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