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PMID: 2034653 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

iciA, an Escherichia coli gene encoding a specific inhibitor of chromosomal initiation of replication in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 10 ·1991-05-15 ·Pages 4066-70

Thöny B, Hwang DS, Fradkin L, Kornberg A

Abstract

The gene encoding the protein that binds the three 13-mers in the origin (oriC) of Escherichia coli to block initiation of replication in vitro has been cloned, sequenced, and overexpressed. The gene possesses an open reading frame for 297 amino acids (mass of 33,471 Da). The protein has a motif for DNA-binding (helix-turn-helix) and has homology to a diverse set of prokaryotic regulatory proteins, known as the LysR family. The protein, previously referred to as the 33-kDa protein, has been named IciA (for inhibitor of chromosome initiation). The iciA gene is at 62.8 min on the chromosomal map. Cells with enhanced levels of the protein grow at a normal rate but generally exhibit a pronounced lag upon transfer to a fresh medium.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Chromosomes, Bacterial Cloning, Molecular DNA Replication DNA-Binding Proteins/genetics Escherichia coli/genetics Escherichia coli Proteins Gene Expression Genes, Bacterial Molecular Sequence Data Nucleic Acid Hybridization Polymerase Chain Reaction Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins argP protein, E coli
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Thöny B
Department of Biochemistry, Stanford University School of Medicine, CA 94305-5307.
Hwang D S
Fradkin L
Kornberg A
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.10.4066
Published
1991-05-15
Pages
4066-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51598
Subset
IM
Grants
NIGMS NIH HHS · GM07581 · United States
Databases
GENBANK
M62865, M64435, M68846, M68847, M68848, M68849, M68850, M68851, M68852, M69013
External Links
PubMed source DOI: 10.1073/pnas.88.10.4066 PMC Full Text View journal details
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