| GeneLibs

题目:: Endothelial cells create a hematopoietic inductive microenvironment preferential to erythropoiesis in the mouse spleen.
作者:: Yanai(N),Satoh(T),Obinata(M)
状态:: 发布时间1991-06-26 , 更新时间 2008-11-21
期刊:: Cell Struct Funct
摘要:: The spleen is an erythropoietic organ in mouse. To reconstruct a microenvironment essential for erythropoiesis in vitro, the stroma (MSS31) cell line had been established from a newborn mouse spleens. MSS31 cells exhibited properties of endothelial cells: (a) the cells showed the activity to uptake acetylated low-density lipoprotein (Ac-LDL) and (b) the cells can form a capillarylike structure by a phenotypic modulation in collagen matrices. MSS31 cells selectively supported the proliferation and differentiation of the erythroid progenitor cells by direct cell-to-cell contact in a semisolid medium in the presence of erythropoietin. These layers also supported erythrocyte maturation and enucleation of erythroblasts. This suggests that spleen endothelial cells are a new type of stromal cell with erythropoietic stimulation activity and may have a critical function in the hemopoietic inductive microenvironment of the mouse spleen.
语言:: eng
DOI:

文献库文献相关信息